Seed vigour is an imperative trait for the direct seeding of rice. In this study, we examined the genetic regulation of seedling percentage at the early germination using a genome-wide association study in rice. One major quantitative trait loci qSP3 for seedling percentage was identified, and the candidate gene was validated as qSP3, encoding a cupin domain protein OsCDP3.10 for the synthesis of 52 kDa globulin. Disruption of this gene in Oscdp3.10 mutants reduced the seed vigour, including the germination potential and seedling percentage, at the early germination in rice. The lacking accumulation of 52 kDa globulin was observed in the mature grains of the Oscdp3.10 mutants. The significantly lower amino acid contents were observed in the mature grains and the early germinating seeds of the Oscdp3.10 mutants compared with those of wild-type. Rice OsCDP3.10 regulated seed vigour mainly via modulating the amino acids e.g. Met, Glu, His, and Tyr that contribute to hydrogen peroxide (H₂O₂) accumulation in the germinating seeds. These results provide important insights into the application of seed priming with the amino acids and the selection of OsCDP3.10 to improve seed vigour in rice.

中文翻译：

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全基因组关联研究表明，cupin结构域蛋白OsCDP3.10调节水稻种子活力

种子活力是水稻直播的必要性状。在这项研究中，我们使用水稻全基因组关联研究检查了萌发早期幼苗百分比的遗传调控。鉴定了一个用于幼苗百分比的主要数量性状基因座qSP3，候选基因被验证为qSP3 ，编码用于合成 52 kDa 球蛋白的 cupin 结构域蛋白OsCDP3.10 。Oscdp3.10突变体中该基因的破坏降低了水稻萌发早期的种子活力，包括发芽潜力和幼苗百分比。在Oscdp3.10的成熟籽粒中观察到缺乏 52 kDa 球蛋白的积累突变体。与野生型相比，Oscdp3.10突变体成熟籽粒和早期萌发种子中氨基酸含量显着降低。水稻OsCDP3.10主要通过调节氨基酸如Met、Glu、His和Tyr来调节种子活力，这些氨基酸有助于过氧化氢(H ₂ O ₂ )在发芽种子中的积累。这些结果为应用氨基酸引发种子和选择OsCDP3.10以提高水稻种子活力提供了重要的见解。