Horseradish peroxidase (HRP) was immobilized in calcium alginate beads by extruding an alginate/HRP solution through a syringe onto calcium chloride. The aim of this study was to evaluate their use to remove the azo-dye Orange II (OII), under different experimental conditions, such as the number of beads, pH, reuse of the biocatalyst, hydrogen peroxide feeding strategy. A mathematical model that takes into account the diffusion of reactants, products, and HRP throughout the beads and the effect of the enzyme leakage on the observable oxidation rate was also developed. Results indicated that the immobilization efficiency decreased as a function of the incubation time in CaCl₂. Although enzyme-containing beads had a low OII adsorption capacity, under the presence of hydrogen peroxide, the dye removal was highly increased due to the catalytic activity of HRP. Besides, the initial OII oxidation rate (R_S0) using 3 beads at pH 9 was higher than at pH 7. Also, R_S0 increased as a function of the number of tested beads. The developed model was fitted to experimental data obtained at different conditions. Then, the model was validated against a new data set that was not used for the calibration of the model, obtaining a satisfactory agreement between simulation results and these new data. Simulations demonstrate that 70–90% of the total OII removed was due to its oxidation by hydrogen peroxide catalyzed by HRP in the liquid phase.

辣根过氧化物酶 (HRP) 通过注射器将海藻酸盐/HRP 溶液挤出到氯化钙上，从而将其固定在海藻酸钙珠中。本研究的目的是评估它们在不同实验条件下（例如珠子数量、pH、生物催化剂的再利用、过氧化氢进料策略）去除偶氮染料橙 II (OII) 的用途。还开发了一个数学模型，该模型考虑了反应物、产物和 HRP 在整个珠子中的扩散以及酶泄漏对可观察氧化速率的影响。结果表明，固定效率随着在 CaCl _{2 中}孵育时间的增加而降低. 尽管含酶珠的 OII 吸附能力较低，但在过氧化氢存在下，由于 HRP 的催化活性，染料去除率大大提高。此外，在 pH 9 下使用 3 个珠子的初始 OII 氧化速率 (R _S0 ) 高于 pH 7。此外，R _S0作为测试珠子数量的函数而增加。开发的模型适合在不同条件下获得的实验数据。然后，针对未用于模型校准的新数据集验证模型，在模拟结果和这些新数据之间获得令人满意的一致性。模拟表明，去除的总 OII 的 70-90% 是由于其在液相中被 HRP 催化的过氧化氢氧化所致。