Pesticide residue contamination has become an urgent issue since it threatens both the natural environment and public health. In this study, a fluorescent method for detecting dithiocarbamate (DTC) compounds was constructed based on novel nickel nanoclusters (Ni NCs) and copper ions (Cu²⁺). The water-soluble fluorescent Ni NCs were synthesized for the first time through a one-pot method using glutathione as stabilizer and ascorbic acid as reducing agent. The as-prepared Ni NCs exhibited a maximum fluorescence emission at 445 nm when excited by 380 nm. And they displayed aggregation-induced emission enhancement when ethylene glycol was introduced into the nanocluster aqueous solution. Based on the Ni NCs, a label-free fluorescence quenching sensor was established for sensitive and selective detection of DTC compounds with the assistance of Cu²⁺. The complex formed by DTC and Cu²⁺ led to fluorescence quenching of Ni NCs through inner filter effect. The sensing method was successfully applied to two typical DTC compounds, thiram and disulfiram, with good linearity over a wide linear range and a low detection limit. Moreover, the proposed approach was capable of thiram detection in real samples, which confirms the potential of this sensing method as a platform for DTC compound detection.

农药残留污染已成为一个紧迫的问题，因为它威胁到自然环境和公众健康。本研究基于新型镍纳米团簇（Ni NCs）和铜离子（Cu ²⁺）构建了一种检测二硫代氨基甲酸盐（DTC）化合物的荧光方法。首次以谷胱甘肽为稳定剂，抗坏血酸为还原剂，通过一锅法合成了水溶性荧光镍纳米晶。 当被 380 激发时，所制备的 Ni NCs 在 445 nm 处表现出最大的荧光发射 纳米。当乙二醇被引入纳米团簇水溶液时，它们表现出聚集诱导的发射增强。基于Ni NCs，建立了一种无标记荧光猝灭传感器，用于在Cu ²⁺的辅助下灵敏、选择性地检测DTC化合物。DTC和Cu ²⁺形成的络合物通过内滤效应导致Ni NCs的荧光猝灭。该传感方法成功应用于两种典型的DTC化合物，福美双和双硫仑，在较宽的线性范围内具有良好的线性和较低的检测限。此外，所提出的方法能够在实际样品中检测福美双，这证实了这种传感方法作为 DTC 化合物检测平台的潜力。