Arterial Internal Elastic Lamina-Inspired Membrane for Providing Biochemical and Structural Cues in Developing Artery-on-a-Chip,ACS Macro Letters

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Arterial Internal Elastic Lamina-Inspired Membrane for Providing Biochemical and Structural Cues in Developing Artery-on-a-Chip
ACS Macro Letters ( IF 5.1 ) Pub Date : 2021-10-20 , DOI: 10.1021/acsmacrolett.1c00551
Jaeseung Youn ₁ , Hyeonseok Han ₁ , Sang Min Park ₂ , Dong Sung Kim _{1,

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Affiliation

In vitro artery models constructed on a membrane-based microfluidic chip, called an artery-on-a-chip, have been spotlighted as a powerful platform for studying arterial physiology. However, due to the use of a flat and porous membrane that cannot mimic the in vivo internal elastic lamina (IEL), the physiological similarity in the phenotypes and the arrangements of the endothelial cells (ECs) and aortic smooth muscle cells (AoSMCs) has been limited in the previously developed artery-on-a-chips. Herein, we developed an innovative membrane mimicking the structures of IEL by utilizing electrospun aligned silk fibroin/polycaprolactone nanofiber membranes. An arterial IEL-mimicking (AIM) membrane was about 5 μm thick and composed of orthogonally aligned nanofibers with a diameter of around 400 nm, which were highly comparable to the IEL. Such structural similarity was found to induce the ECs and SMCs to be elongated and orthogonally aligned as in the in vivo artery. In particular, the SMCs cultured on the AIM membrane maintained a healthy state showing increased αSMA mRNA expression, which was easily lost on the conventional membrane. We constructed an AIM membrane-integrated artery-on-a-chip having an orthogonal arrangement of ECs and SMCs, which was desirable but difficult to be realized with the previous artery-on-a-chip.

中文翻译：

动脉内弹性层膜为开发芯片上的动脉提供生化和结构线索

在基于膜的微流控芯片（称为芯片上的动脉）上构建的体外动脉模型已成为研究动脉生理学的强大平台。然而，由于使用了无法模拟体内的扁平多孔膜内弹力层 (IEL)、表型的生理相似性以及内皮细胞 (EC) 和主动脉平滑肌细胞 (AoSMC) 的排列在先前开发的动脉芯片中受到限制。在这里，我们开发了一种创新的膜，通过利用静电纺丝排列的丝素蛋白/聚己内酯纳米纤维膜来模拟 IEL 的结构。动脉 IEL 模拟 (AIM) 膜厚约 5 μm，由直径约 400 nm 的正交排列的纳米纤维组成，与 IEL 高度可比。发现这种结构相似性会导致 ECs 和 SMCs 像体内一样被拉长和正交排列动脉。特别是，在 AIM 膜上培养的 SMC 保持健康状态，显示出增加的 αSMA mRNA 表达，这在传统膜上很容易丢失。我们构建了一个 AIM 膜集成动脉芯片，具有 EC 和 SMC 的正交排列，这是可取的，但很难用以前的芯片动脉实现。

更新日期：2021-11-16

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