Antimicrobial peptides (AMPs) have been reported to be promising alternatives to chemical preservatives. Thus, this study aimed to characterise AMPs generated from the hydrolysis of wheat gluten proteins using latex peptidases of Calotropis procera, Cryptostegia grandiflora, and Carica papaya. The three hydrolysates (obtained after 16 h at 37 °C, using a 1: 25 enzyme: substrate ratio) inhibited the growth of Aspergillus niger, A. chevalieri, Trichoderma reesei, Pythium oligandrum, Penicillium sp., and Lasiodiplodia sp. by 60–90%, and delayed fungal growth on bread by 3 days when used at 0.3 g/kg. Moreover, the specific volume and expansion factor of bread were not affected by the hydrolysates. Of 28 peptides identified, four were synthesised and exhibited activity against Penicillium sp. Fluorescence and scanning electron microscopy suggested that the peptides damaged the fungal plasma membrane. Bioinformatics analysis showed that no peptide was toxic and that the antigenic ones had cleavage sites for trypsin or pepsin.

据报道，抗菌肽 (AMP) 是化学防腐剂的有前途的替代品。因此，本研究旨在表征使用Calotropis procera、Cryptostegia grandiflora和Carica papaya 的乳胶肽酶水解小麦面筋蛋白产生的 AMP 。三个水解物（在37℃下16小时后获得，使用1：25的酶：底物比例）抑制的生长黑曲霉，A.薛氏，里氏木霉，寡雄腐霉，青霉属，和。Lasiodiplodiasp. 降低 60-90%，并在以 0.3 g/kg 使用时将面包上的真菌生长延迟 3 天。此外，面包的比体积和膨胀系数不受水解产物的影响。在鉴定的 28 种肽中，合成了 4 种并表现出抗青霉菌的活性。荧光和扫描电子显微镜表明这些肽破坏了真菌质膜。生物信息学分析表明，没有肽是有毒的，抗原肽具有胰蛋白酶或胃蛋白酶的裂解位点。