This study investigated for the first time if ammonia tolerant methanogenic consortia can be stored in gel (biogel) and used in a later time on-demand as bioaugmentation inocula, to efficiently relieve ammonia inhibition in continuous biomethanation systems. Moreover, wood biochar was assessed as a potential enhancer of the novel biogel bioaugmentation process. Three thermophilic (55 °C), continuous stirred-tank reactors (R_Bgel, R_Char and R_Mix), operated at 4.5 g NH₄⁺-N L^-1 were exposed to biogel, biochar and mixture of biogel and biochar, respectively, while a fourth reactor (R_Ctrl) was used as control. The results showed that the methane production yields of R_Mix, R_Char and R_Bgel increased by 28.6%, 20.2% and 10.7%, respectively compared to R_Ctrl. The highest methane yield was achieved by the synergistic interaction between biogel and biochar. Additionally, biogel stimulated a rapid recovery of Methanoculleus thermophilus sp. and syntrophic acetate oxidising bacteria populations.

该研究首次调查了耐氨产甲烷菌群是否可以储存在凝胶（生物凝胶）中，并在以后按需用作生物强化接种物，以有效缓解连续生物甲烷化系统中的氨抑制。此外，木材生物炭被评估为新型生物凝胶生物强化过程的潜在增强剂。三个嗜热（55℃），连续搅拌釜式反应器（R _Bgel，R_字符和R_混合），在操作4.5克NH ₄ ⁺ -NL ^-1分别暴露于生物胶，生物炭和生物凝胶和生物炭，混合物，而第四个反应器 (R _Ctrl ) 用作对照。结果表明，R的甲烷产率与 R _Ctrl相比，_Mix、R _Char和 R _{Bgel 分别}增加了 28.6%、20.2% 和 10.7% 。最高的甲烷产量是通过生物凝胶和生物炭之间的协同相互作用实现的。此外，生物凝胶刺激了嗜热甲烷菌的快速恢复。和同养醋酸盐氧化菌群。