Two consortia of lignocellulolytic microbes (CL and YL) were isolated from the rumen of ruminants. Their ability to facilitate the degradation of rice straw and enhance methane (CH₄) production were evaluated, both individually and combined with lactic acid bacteria (LAB). After 30 days of degradation, rice straw powders (RSPs) were observed to change in physical structure and also displayed a significant reduction in lignocellulose content. Combined application of microbial consortia with LAB efficiently improved enzymatic hydrolysis of RSPs, increasing organic acid as well as mono- and disaccharide contents. Synergistic action between microbial consortia and LAB enhanced CH₄ yield, and rice straw treated with YL + LAB had the highest CH₄ production (357.53 mL CH₄/g VS), more than fivefold of the control. The newly identified microbial consortia are capable of efficiently degrading lignocellulosic biomass. Functioning synergistically with LAB, they provide a feasible way biodegrade rice straw and enhance methane production from agricultural wastes.

从反刍动物的瘤胃中分离出两个木质纤维素分解微生物群落（CL 和 YL）。评估了它们促进稻草降解和提高甲烷 (CH ₄ ) 产生的能力，无论是单独使用还是与乳酸菌 (LAB) 结合使用。降解 30 天后，观察到稻草粉 (RSP) 的物理结构发生了变化，并且木质纤维素含量也显着降低。微生物菌群与 LAB 的联合应用有效地改善了 RSP 的酶水解，增加了有机酸以及单糖和二糖的含量。微生物聚生体与LAB增强CH之间的协同动作₄产率，和稻草与YL + LAB处理具有最高的CH ₄产量（357.53 mL CH ₄ /g VS），是对照的五倍多。新发现的微生物聚生体能够有效降解木质纤维素生物质。它们与 LAB 协同发挥作用，提供了一种可行的方法来生物降解稻草并提高农业废物中的甲烷产量。