The present work shows the results of optimized alkaline pretreatment and enzymatic hydrolysis processes for the production of bioethanol from cocoa pod husk (CPH). Alkaline pretreatments were applied to evaluate the effect of NaOH concentration, residence time, and temperature using a Central Composite Design (CCD). SEM and XRD methodologies were used to study the structural characteristics of the solid fraction. Enzymatic hydrolysis assays were applied to optimize the enzyme (Cellic Ctec2, Novozyme) and solid loadings. The hydrolysate was subjected to fermentation using the yeast Saccharomyces cerevisiae Y2034. The results showed that the optimal condition for the alkaline pretreatment was 5% (w v⁻¹) NaOH for 30 min at 120 °C, which increased the cellulose content of CPH from 27.68 ± 0.15 (untreated) to 57 ± 0.25%. SEM analysis showed changes in porosity and alterations in the biomass, while the XRD revealed an increase in crystallinity. The enzymatic hydrolysis was optimized to 10 FPU g⁻¹ substrate and solid loading of 10% (w v⁻¹), obtaining yields of 98.75%, equivalent to 66.80 g L⁻¹ reducing sugars. During fermentation, 80.74% of reducing sugars were consumed, producing 18.06 g L⁻¹ of ethanol in 24 h, corresponding to a theoretical yield of 51.45%. The results obtained show the potential of the use of cocoa pod husk as raw material for the production of bioethanol.

目前的工作显示了从可可豆壳 (CPH) 生产生物乙醇的优化碱性预处理和酶水解工艺的结果。使用中央复合设计 (CCD) 应用碱性预处理来评估 NaOH 浓度、停留时间和温度的影响。SEM和XRD方法用于研究固体部分的结构特征。应用酶水解测定来优化酶（Cellic Ctec2，Novozyme）和固体负载。使用酵母Saccharomyces cerevisiae Y2034对水解产物进行发酵。结果表明，碱性预处理的最佳条件为 5% (wv ^-1) NaOH 在 120 °C 下保持 30 分钟，这将 CPH 的纤维素含量从 27.68 ± 0.15（未处理）增加到 57 ± 0.25%。SEM 分析显示生物质的孔隙率发生变化和改变，而 XRD 显示结晶度增加。酶水解被优化为10 FPU g ^-1底物和10% (wv ^-1 )的固体负载，获得98.75%的产率，相当于66.80 g L ^-1还原糖。在发酵过程中，80.74%的还原糖被消耗，24小时内产生18.06 g L ^-1乙醇，对应的理论产率为51.45%。获得的结果显示了使用可可豆壳作为生产生物乙醇的原料的潜力。