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Acoustofluidic Droplet Sorter Based on Single Phase Focused Transducers
Small ( IF 13.0 ) Pub Date : 2021-10-17 , DOI: 10.1002/smll.202103848
Ruoyu Zhong 1 , Shujie Yang 1 , Giovanni Stefano Ugolini 2 , Ty Naquin 1 , Jinxin Zhang 1 , Kaichun Yang 1 , Jianping Xia 1 , Tania Konry 2 , Tony Jun Huang 1
Affiliation  

Droplet microfluidics has revolutionized the biomedical and drug development fields by allowing for independent microenvironments to conduct drug screening at the single cell level. However, current microfluidic sorting devices suffer from drawbacks such as high voltage requirements (e.g., >200 Vpp), low biocompatibility, and/or low throughput. In this article, a single-phase focused transducer (SPFT)-based acoustofluidic chip is introduced, which outperforms many microfluidic droplet sorting devices through high energy transmission efficiency, high accuracy, and high biocompatibility. The SPFT-based sorter can be driven with an input power lower than 20 Vpp and maintain a postsorting cell viability of 93.5%. The SPFT sorter can achieve a throughput over 1000 events per second and a sorting purity up to 99.2%. The SPFT sorter is utilized here for the screening of doxorubicin cytotoxicity on cancer and noncancer cells, proving its drug screening capability. Overall, the SPFT droplet sorting device shows great potential for fast, precise, and biocompatible drug screening.

中文翻译:


基于单相聚焦传感器的声流控液滴分选器



液滴微流控允许独立的微环境在单细胞水平上进行药物筛选,彻底改变了生物医学和药物开发领域。然而,当前的微流体分选装置存在诸如高电压要求(例如,>200Vpp)、低生物相容性和/或低吞吐量等缺点。本文介绍了一种基于单相聚焦换能器(SPFT)的声流控芯片,该芯片具有高能量传输效率、高精度和高生物相容性,优于许多微流控液滴分选装置。基于 SPFT 的分选机可以用低于 20 Vpp 的输入功率驱动,并保持分选后细胞活力为 93.5%。 SPFT分选机每秒可处理超过1000个事件,分选纯度高达99.2%。 SPFT分选仪用于筛选阿霉素对癌细胞和非癌细胞的细胞毒性,证明了其药物筛选能力。总体而言,SPFT 液滴分选装置在快速、精确和生物相容性药物筛选方面显示出巨大的潜力。
更新日期:2021-11-18
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