Manipulating the Assembly of DNA Nanostructures and Their Enzymatic Properties by Incorporating a 5′-5′ Polarity of Inversion Site in the G-Tract,ACS Macro Letters

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Manipulating the Assembly of DNA Nanostructures and Their Enzymatic Properties by Incorporating a 5′-5′ Polarity of Inversion Site in the G-Tract
ACS Macro Letters ( IF 5.1 ) Pub Date : 2021-10-18 , DOI: 10.1021/acsmacrolett.1c00490
Yanwei Cao ₁ , Wenjing Li ₁ , Renjun Pei ₁

Affiliation

Supramolecular DNA complexes consisting of both DNA duplexes and tetrameric G-quadruplexes are fabricated successfully by utilizing a single short DNA strand that contains one 5′-5′ polarity of inversion site in the middle of G-tract. The resulting DNA supramolecules exhibit significantly high peroxidase activities after interaction with hemin due to the presence of various G-quadruplex–duplex (G4–duplex) interfaces. Significantly, we find that the addition of a C-rich fragment to the designed sequence not only allows the self-assembly of two-dimensional porous DNA nanostructures via the formation of dimeric i-motif structures but also could act as a control element to facilitate the generation of pH-sensitive G4-based DNAzymes. The enhanced catalytic activity obtained from specific sequence modifications as well as the controllable feature of these DNA nanostructures can significantly benefit further applications of DNA functional materials in complex biological systems.

中文翻译：

通过在 G-Tract 中引入 5'-5' 反转位点极性来操纵 DNA 纳米结构的组装及其酶学性质

由 DNA 双链体和四聚体 G-四链体组成的超分子 DNA 复合物是通过利用在 G 道中间含有一个 5'-5' 极性反转位点的单个短 DNA 链成功制造的。由于存在各种 G-四链体-双链体 (G4-双链体) 界面，所得 DNA 超分子在与血红素相互作用后表现出显着高的过氧化物酶活性。重要的是，我们发现在设计的序列中添加富含 C 的片段不仅可以通过形成二聚体 i 基序结构自组装二维多孔 DNA 纳米结构，而且还可以作为控制元件促进pH敏感的基于G4的脱氧核糖核酸酶的产生。

更新日期：2021-11-16

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