Identifying individuals by their natural body markings is an excellent surrogate for artificial tags. Photo identification has been primarily tested in oceanic species but has not been extensively used on freshwater fish. Brook trout are renowned for their lateral spot patterns. In this study, Interactive Individual Identification System was used to digitize and assess spot pattern uniqueness for identifying individual brook trout within a population for a short-term mark-recapture study. Brook trout were sampled over multiple days in Harper Creek and captured 315 specimens. Photo images were collected of their left lateral side and digitized. Based on photo identification, 263 unique brook trout were sampled. Additionally, there were 36 recaptures: 29 sampled twice, six sampled three times, and one sampled four times enabling a population estimate to be derived. In 2019, Harper Creek supported a spawning population of 514.4 (408–659; 95% CIs) brook trout. Ninety-four unique brook trout were sampled from a geographically distinct population of which none of the spot patterns matched the Harper Creek population. Spot patterns were distinct between lateral sides of an individual fish. Spot patterns on brook trout are unique and can be used to as a non-invasive means to identify individual at least in short-term projects.

通过自然的身体标记来识别个人是人工标签的绝佳替代品。照片识别主要在海洋物种中进行了测试，但尚未广泛用于淡水鱼。布鲁克鳟鱼以其横向斑点图案而闻名。在这项研究中，交互式个体识别系统用于数字化和评估斑点模式的独特性，以识别种群中的个体鳟鱼，以进行短期标记重新捕获研究。布鲁克鳟鱼在 Harper Creek 进行了多天的采样，并捕获了 315 个标本。收集他们左侧的照片图像并进行数字化。根据照片鉴定，抽取了 263 条独特的鳟鱼。此外，还有 36 次重新捕获：29 次采样两次，6 次采样三次，并且其中一个采样四次，从而可以得出总体估计值。2019 年，Harper Creek 支持了 514.4（408-659；95% CI）鳟鱼的产卵种群。从地理上不同的种群中抽取了 94 条独特的鳟鱼，其中没有一个斑点模式与哈珀溪种群相匹配。单个鱼的侧面之间的斑点图案是不同的。鳟鱼上的斑点图案是独一无二的，至少在短期项目中可用作识别个体的非侵入性手段。单个鱼的侧面之间的斑点图案是不同的。鳟鱼上的斑点图案是独一无二的，至少在短期项目中可用作识别个体的非侵入性手段。单个鱼的侧面之间的斑点图案是不同的。鳟鱼上的斑点图案是独一无二的，至少在短期项目中可用作识别个体的非侵入性手段。