Iso-nitrogenous and iso-lipidic diets containing 0%, 3%, 6%, 9%, and 12% hydrolyzed porcine mucosa (namely, HPM0, HPM3, HPM6, HPM9, and HPM12) were prepared to evaluate their effects on the growth performance, muscle nutrition composition, texture property, and gene expression related to muscle growth of hybrid groupers (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂). Groupers were fed to apparent satiation at 08:00 and 16:00 every day for a total of 56 days. It was found that the weight gain percentage in the HPM0, HPM3, and HPM6 groups did not differ (P > 0.05). The cooking loss and drip loss of the dorsal muscle in the HPM3 group were lower than those in the HPM6 and HPM9 groups (P < 0.05). The hardness and chewiness of the dorsal muscle in the HPM3 group were higher than those in the HPM0, HPM9, and HPM12 groups (P < 0.05). The gumminess in the HPM3 group was higher than that in the HPM9 and HPM12 groups (P < 0.05). The total essential amino acid content of the dorsal muscle in the HPM12 group was higher than that in the HPM0 group (P < 0.05). The contents of total n-3 polyunsaturated fatty acid and total n-3 highly unsaturated fatty acid, as well as the ratio of n-3/n-6 polyunsaturated fatty acid in the dorsal muscle was higher in the HPM0 group than in all other groups (P < 0.05). The relative expressions of gene myogenic factor 5, myocyte enhancer factor 2c, myocyte enhancer factor 2a, myosin heavy chain, transforming growth factor-beta 1 (TGF-β1), and follistatin (FST) were the highest in the dorsal muscle of the HPM3 group. The results indicated that the growth performance of hybrid grouper fed a diet with 6% HPM and 27% fish meal was as good as that of the HPM0 group. When fish ingested a diet containing 3% HPM, the expression of genes TGF-β1 and FST involved in muscle growth were upregulated, and then the muscle quality related to hardness and chewiness were improved. An appropriate amount of HPM could be better used in grouper feed.

制备含有0%、3%、6%、9%和12%水解猪粘膜（即HPM0、HPM3、HPM6、HPM9和HPM12）的等氮和等脂日粮，以评估它们对生长的影响杂种石斑鱼（Epinephelus fuscoguttatus ♀ ×  Epinephelus lanceolatus ♂）的性能、肌肉营养成分、质地特性和与肌肉生长相关的基因表达。每天 08:00 和 16:00 将石斑鱼喂至明显饱食状态，共 56 天。发现HPM0、HPM3和HPM6组的体重增加百分比没有差异（P  > 0.05）。HPM3组背肌蒸煮损失和滴水损失低于HPM6和HPM9组（P < 0.05)。HPM3组背肌硬度和咀嚼性高于HPM0、HPM9和HPM12组（P  < 0.05）。HPM3组的胶粘性高于HPM9和HPM12组（P  < 0.05）。HPM12组背肌总必需氨基酸含量高于HPM0组（P  < 0.05）。HPM0组背肌总n-3多不饱和脂肪酸和总n-3高度不饱和脂肪酸含量以及n-3/n-6多不饱和脂肪酸比均高于其他组组（P < 0.05)。基因生肌因子5、肌细胞增强因子2c、肌细胞增强因子2a、肌球蛋白重链、转化生长因子-β1（TGF - β1）和卵泡抑素（FST）的相对表达在HPM3背肌中最高团体。结果表明，饲喂含 6% HPM 和 27% 鱼粉的日粮的杂交石斑鱼的生长性能与 HPM0 组一样好。当鱼摄入含 3% HPM 的饲料时，参与肌肉生长的基因TGF-β1和FST 的表达上调，进而改善与硬度和咀嚼性相关的肌肉质量。适量的HPM可更好地用于石斑鱼饲料。