Pseudomonas cannabina pv. alisalensis is a causative agent of bacterial blight of crucifers including cabbage, radish, and broccoli. Importantly, P. cannabina pv. alisalensis can infect not only a wide range of Brassicaceae spp. but, also, green manure crops such as oat. However, P. cannabina pv. alisalensis virulence mechanisms have not been investigated and are not fully understood. We focused on coronatine (COR) function, which is one of the well-known P. syringae pv. tomato DC3000 virulence factors, in P. cannabina pv. alisalensis infection processes on both dicot and monocot plants. Cabbage and oat plants dip-inoculated with a P. cannabina pv. alisalensis KB211 COR mutant (ΔcmaA) exhibited reduced virulence compared with P. cannabina pv. alisalensis wild type (WT). Moreover, ΔcmaA failed to reopen stomata on both cabbage and oat, suggesting that COR facilitates P. cannabina pv. alisalensis entry through stomata into both plants. Furthermore, cabbage and oat plants syringe-infiltrated with ΔcmaA also showed reduced virulence, suggesting that COR is involved in overcoming not only stomatal-based defense but also apoplastic defense. Indeed, defense-related genes, including PR1 and PR2, were highly expressed in plants inoculated with ΔcmaA compared with WT, indicating that COR suppresses defense-related genes of both cabbage and oat. Additionally, salicylic acid accumulation increases after ΔcmaA inoculation compared with WT. Taken together, COR contributes to causing disease by suppressing stomatal-based defense and apoplastic defense in both dicot and monocot plants. Here, we investigated COR functions in the interaction of P. cannabina pv. alisalensis and different host plants (dicot and monocot plants), using genetically and biochemically defined COR deletion mutants.

The author(s) have dedicated the work to the public domain under the Creative Commons CC0 “No Rights Reserved” license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2021.

假单胞菌大麻光伏。alisalensis是十字花科植物细菌性枯萎病的病原体，包括卷心菜、萝卜和西兰花。重要的是，P.大麻光伏。alisalensis 不仅可以感染广泛的十字花科植物。但是，还有绿肥作物，如燕麦。然而，P.大麻光伏。alisalensis 的毒力机制尚未得到研究，也未完全了解。我们专注于coronatine (COR) 功能，这是著名的丁香假单胞菌光伏发电之一。番茄DC3000 毒力因子，在P. cannabina pv。大丽花双子叶植物和单子叶植物的感染过程。用P. cannabina pv浸渍接种的卷心菜和燕麦植物。与P. cannabina pv相比，alisalensis KB211 COR 突变体 (Δ cmaA ) 表现出降低的毒力。alisalensis野生型 (WT)。此外，Δ cmaA未能重新打开卷心菜和燕麦上的气孔，表明 COR 促进P. cannabina pv。alisalensis通过气孔进入两种植物。此外，甘蓝和燕麦植物注射器渗入了 Δ cmaA还显示毒力降低，表明 COR 不仅参与克服基于气孔的防御，还参与克服质外体防御。事实上，与 WT 相比，包括PR1和PR2在内的防御相关基因在接种了 Δ cmaA 的植物中高度表达，表明 COR 抑制了卷心菜和燕麦的防御相关基因。此外，与 WT 相比，接种Δ cmaA后水杨酸的积累增加。总之，COR 通过抑制双子叶植物和单子叶植物中基于气孔的防御和质外体防御而导致疾病。在这里，我们研究了P. cannabina pv相互作用中的 COR 函数。大丽花 和不同的寄主植物（双子叶植物和单子叶植物），使用遗传和生化定义的 COR 缺失突变体。

作者已根据 Creative Commons CC0“No Rights Reserved”许可将作品献给公有领域，在一定程度上放弃了他或她根据版权法在全球范围内对作品的所有权利，包括所有相关和邻接权法律允许，2021 年。