Identification of transcription factor binding sites is one of the most important steps in understanding the function of transcription factors and regulatory networks in organisms. The assay for transposase accessible chromatin sequencing (ATAC-seq) is a simple protocol for detection of open chromatin that could be a powerful tool to advance studies of protein-DNA interactions. Although ATAC-seq has been used in systematic identification of cis-regulatory regions in animal and plant genomes, this method has been rarely applied in fungi. Here, we describe a valuable ATAC-seq resource in the genome of an economically important phytopathogen, the rice false smut fungus Ustilaginoidea virens. The ATAC-seq data of U. virens mycelia collected from potato sucrose broth (PSB) and PSB supplied with rice spikelet extract were both generated. This is the first genome-wide profiling of open chromatin and transcription factor binding sites in U. virens.

Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

转录因子结合位点的鉴定是了解生物体中转录因子和调节网络功能的最重要步骤之一。转座酶可及染色质测序 (ATAC-seq) 检测是一种检测开放染色质的简单方案，可以成为推进蛋白质-DNA 相互作用研究的有力工具。尽管 ATAC-seq 已用于系统鉴定动植物基因组中的顺式调控区域，但该方法很少应用于真菌。在这里，我们描述了一种具有重要经济意义的植物病原体——水稻假黑穗病真菌Ustilaginoidea virens基因组中的宝贵 ATAC-seq 资源。U. virens的 ATAC-seq 数据从马铃薯蔗糖肉汤 (PSB) 中收集的菌丝体和用水稻小穗提取物提供的 PSB 都产生了。这是开放的染色质和转录因子结合位点的第一个全基因组分析稻曲病菌。

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