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Improved Whole-Genome Sequence of Phytophthora capsici Generated by Long-Read Sequencing
Molecular Plant-Microbe Interactions ( IF 3.2 ) Pub Date : 2021-08-24 , DOI: 10.1094/mpmi-12-20-0356-a
Jinxia Shi 1 , Wenwu Ye 2 , Dongfang Ma 3 , Junliang Yin 3 , Zhichao Zhang 4 , Yuanchao Wang 5 , Yongli Qiao 6
Affiliation  

The soilborne oomycete Phytophthora capsici is the most destructive pathogen of vegetable crops and is responsible for substantial economic losses worldwide. Here, we present an improved genome assembly of P. capsici generated by Oxford Nanopore long-read sequencing (for de novo assembly) and Illumina short-read sequencing (for polishing). The genome of P. capsici is 100.5 Mb in length (GC content = 50.8%) and contains 26,069 predicted protein-coding genes. The whole genome of P. capsici is assembled into 194 scaffolds, 90% of which are larger than 300 kb. The N50 scaffold length and maximum scaffold length are 1.0 and 4.1 Mb, respectively. The whole-genome sequence of P. capsici will broaden our knowledge of this pathogen and enhance our understanding of the molecular basis of its pathogenicity, which will facilitate the development of effective management strategies.

Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.



中文翻译:

长读长测序产生的辣椒疫霉全基因组序列的改进

土壤传播的卵菌Phytophthora capsici是对蔬菜作物最具破坏性的病原体,在全世界造成了巨大的经济损失。在这里,我们展示了由牛津纳米孔长读长测序(用于从头组装)和 Illumina 短读长测序(用于抛光)生成的辣椒辣椒基因组组装的改进。P. capsici的基因组长度为 100.5 Mb(GC 含量 = 50.8%),包含 26,069 个预测的蛋白质编码基因。P. capsici的全基因组组装成 194 个支架,其中 90% 大于 300 kb。N 50支架长度和最大支架长度分别为 1.0 和 4.1 Mb。辣椒全基因组序列 将拓宽我们对这种病原体的认识,增强我们对其致病性分子基础的理解,这将有助于制定有效的管理策略。

版权所有 © 2021 作者。这是在 CC BY-NC-ND 4.0 国际许可下分发的开放获取文章。

更新日期:2021-10-17
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