Xanthomonas citri pv. punicae causing bacterial blight is a devastating disease of pomegranate in India and Pakistan. Most xanthomonads use the type III secretion system to inject transcription activator-like effector (TALE) proteins into the host cell. TALEs bind to the effector-binding elements in the promoter of host susceptibility genes, triggering disease development. PacBio single-molecule real-time long-read sequencing technology was used to identify the TALE-encoding genes, which is otherwise not possible using next-generation short-read sequencers. In all, 1.74 Gb of raw data containing 368,980 subreads, with an average read length of 4,724 bp and longest read length of 77,471, were generated. Subreads were assembled into 15 scaffolds generating approximately 5.4 Mb (348×) of genome. X. citri pv. punicae exhibited close lineage with X. citri pv. citri with 98.78% average nucleotide identity. Of the 4,263 protein-coding genes, 11 non-TALE type III effectors and 2 TALE-encoding genes were identified.

Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

柑橘黄单胞菌光伏发电。导致细菌性枯萎病的punicae是印度和巴基斯坦石榴的一种毁灭性病害。大多数黄单胞菌使用 III 型分泌系统将转录激活因子样效应 (TALE) 蛋白注入宿主细胞。TALEs 与宿主易感基因启动子中的效应结合元件结合，引发疾病发展。PacBio 单分子实时长读长测序技术用于识别 TALE 编码基因，否则使用下一代短读长测序仪是不可能做到的。总共生成了 1.74 Gb 的原始数据，其中包含 368,980 个子读取，平均读取长度为 4,724 bp，最长读取长度为 77,471。Subreads 被组装到 15 个支架中，产生大约 5.4 Mb (348x) 的基因组。X. 柑橘光伏。punicae与X. citri pv.表现出密切的谱系。citri具有 98.78% 的平均核苷酸同一性。在 4,263 个蛋白质编码基因中，鉴定了 11 个非 TALE III 型效应子和 2 个 TALE 编码基因。

版权所有 © 2021 作者。这是在 CC BY-NC-ND 4.0 国际许可下分发的开放获取文章。