Erythromycin fermentation dregs (EFD) as one kind of organic-rich biosolid was of great potential for methane production. However, the influence of residual erythromycin (ERY) on the anaerobic digestion process of EFD remains unclear. In this study, a batch test was conducted with different ERY concentrations to investigate its effects on methanogenesis. The antibiotic resistance genes and microbial community composition were analyzed to explore the potential mechanism. The results showed that more than 80% of ERY was removed after 30 days digestion. Furthermore, 100, 200 and 300 mg/L of ERY presented no significant effect on the performance of anaerobic digestion. Instead, a high concentration of ERY (500 mg/L) increased 13% rather than inhibited the methane yields. Moreover, the proliferation of the methylase gene (e.g., ermA/T) was promoted under the high pressure of ERY. The relative abundance of acetogenic bacteria (Sedimentibacter) and mixotrophic archaea (Methanosarcina) were enhanced, indicating that their syntrophic association would play the dominant role in the stimulating effects of methanogenesis.

红霉素发酵渣（EFD）作为一种富含有机物的生物固体，具有巨大的产甲烷潜力。然而，残留红霉素 (ERY) 对 EFD 厌氧消化过程的影响仍不清楚。在这项研究中，使用不同的 ERY 浓度进行了批量测试，以研究其对产甲烷的影响。分析抗生素抗性基因和微生物群落组成以探索其潜在机制。结果表明，消化 30 天后，80% 以上的 ERY 被去除。此外，100、200 和 300 mg/L 的 ERY 对厌氧消化性能没有显着影响。相反，高浓度的 ERY (500 mg/L) 增加了 13%，而不是抑制了甲烷产量。此外，甲基化酶基因的增殖（例如，erm A/T) 在 ERY 的高压下得到提升。产乙酸菌（Sedimentibacter）和混合营养古菌（Methanosarcina）的相对丰度增加，表明它们的共养关联在产甲烷作用的刺激作用中起主导作用。