Saline wastewater poses a challenge during bio-treatment process due to salinity affecting the physiological activity of microorganisms and inhibiting their growth and metabolism. Thus, screening and engineering the salt-tolerant strains with stronger performances are urgent. Shewanella aquimarina XMS-1, a salt-tolerant dissimilated metal reducing bacteria (DMRB), was isolated from seawater environment. Its ability for reducing pollutants and generating electricity was enhanced by overexpression of riboflavin synthesis pathway encoding genes from S. oneidensis MR-1 under salt stress. Furthermore, upon contact with graphene oxide (GO), the engineered strain XMS-1/pYYDT-rib with enhanced flavins synthesis could reduce GO and self-assemble to form a three-dimensional (3D) biohybrid system named XMS-1/flavins/rGO. This 3D biohybrid system significantly enhanced the EET efficiency of S. aquimarina XMS-1. Our findings provide a feasible strategy for treatment of salt-containing industrial wastewater contaminated by metal and organic pollutants.

由于盐分会影响微生物的生理活动并抑制其生长和代谢，因此含盐废水在生物处理过程中构成了挑战。因此，筛选和改造具有更强性能的耐盐菌株迫在眉睫。Shewanella aquimarina XMS-1 是一种耐盐异化金属还原菌 (DMRB)，是从海水环境中分离出来的。通过过度表达奥氏沙门氏菌核黄素合成途径编码基因，增强了其减少污染物和发电的能力盐胁迫下的 MR-1。此外，在与氧化石墨烯 (GO) 接触后，具有增强黄素合成的工程菌株 XMS-1/pYYDT-rib 可以减少 GO 并自组装形成名为 XMS-1/flavins/ 的三维 (3D) 生物杂化系统。 rGO。这种 3D 生物杂交系统显着提高了S. aquimarina XMS-1的 EET 效率。我们的研究结果为处理被金属和有机污染物污染的含盐工业废水提供了一种可行的策略。