Abstract

In the green synthesis method, various plants gain importance due to the synergistic effect created by their rich phytochemical properties. In this study, SM (thistle-Silybum marianum) plant, which is known with its antioxidant and anticarcinogenic properties in the literature, forms the source of motivation. Silver nanoparticles (Ag NPs) were synthesized in SM aqueous extracts by this method. The formation of Ag NPs was confirmed by transmission electron microscopy (TEM), UV-Vis and XRD spectrophotometers. Cytotoxicity of MCF-7 cells, incubated for 24 and 48 hours with different mass concentrations of Ag NPs, was determined by MTT test. Additionally, in this study, the radio-sensitizing effects of Ag NPs synthesized via SM seeds were investigated for the first time. Moreover, MCF-7 cells incubated with Ag NPs for 1-2 and 4 hours were exposed to 6 MV energy X-ray radiation.

摘要

在绿色合成方法中，各种植物由于其丰富的植物化学特性所产生的协同效应而变得重要。在这项研究中，SM (thistle- Silybum marianum) 植物，在文献中以其抗氧化和抗癌特性而闻名，构成了动力的来源。通过这种方法在 SM 水提取物中合成银纳米粒子 (Ag NPs)。通过透射电子显微镜 (TEM)、UV-Vis 和 XRD 分光光度计证实了 Ag NPs 的形成。MCF-7 细胞与不同质量浓度的 Ag NPs 孵育 24 和 48 小时后的细胞毒性通过 MTT 测试确定。此外，在这项研究中，首次研究了通过 SM 种子合成的 Ag NPs 的放射增敏作用。此外，将与 Ag NPs 孵育 1-2 小时和 4 小时的 MCF-7 细胞暴露于 6 MV 能量的 X 射线辐射。