Endothelial cells adhere to one another through junctional structures formed by intercellular adhesion molecules. These intercellular proteins regulate barrier function in response to the hemodynamic shear rate and enable the selective passage of solutes and fluids across the endothelium. After endovascular device implantation, the endothelial barrier is compromised and becomes discontinuous, which increases permeability, allowing transmigration of leukocytes and lipoproteins and leading to the accumulation of lipid-laden foamy macrophages in the subendothelial space. Drug-coated bioresorbable vascular scaffold implants have been associated with unexpected thrombotic complications, which were not predicted in animals because of dissimilarities in endothelial regeneration and realignment between animals and humans. The development of a microengineered, microfluidics-based system of patterned channels lined with human endothelial and smooth muscle cells perfused with blood allows for the evaluation of endothelial function and barrier integrity. This review highlights the translational potential of vasculature-on-chip, which recreates the microphysiological milieu to evaluate the impact of drug-eluting bioresorbable vascular scaffolds on endothelial barrier integrity and to characterize polymer biodegradation behavior and drug release kinetic profiles over time.

中文翻译：

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用于评估生物可吸收支架和内皮屏障完整性的芯片血管系统。

内皮细胞通过细胞间粘附分子形成的连接结构相互粘附。这些细胞间蛋白质响应血流动力学剪切速率调节屏障功能，并使溶质和流体能够选择性地穿过内皮。血管内装置植入后，内皮屏障受损并变得不连续，这增加了通透性，允许白细胞和脂蛋白迁移，并导致内皮下空间中富含脂质的泡沫巨噬细胞积累。药物涂层的生物可吸收血管支架植入物与意想不到的血栓并发症有关，由于动物和人类之间内皮再生和重新排列的不同，这在动物身上没有预测到。开发了一种微工程、基于微流体的图案通道系统，该通道内衬人内皮细胞和灌注血液的平滑肌细胞，可以评估内皮功能和屏障完整性。这篇综述强调了芯片上血管系统的转化潜力，它重建了微生理环境，以评估药物洗脱生物可吸收血管支架对内皮屏障完整性的影响，并随着时间的推移表征聚合物生物降解行为和药物释放动力学曲线。