ABSTRACT

The seedless barberry, ‘Bidaneh,’ is one of the most important cultivated small fruits in Iran. This cultivar is propagated only by suckers, and so, the establishment of large-scale orchards is not possible in a short period of time. During this research, different grafting methods were evaluated on the grafting success rate of ‘Bidaneh’ on wild types of barberry. More than 1000 grafts including cleft grafting (with hard shoots and green/semi-hard shoots), omega grafting, T-budding, and chip budding (at mid-spring and late summer) methods were performed for two consecutive years (2018 and 2019). The results showed that the grafting methods have significant effects on the percentage of callus formation and graft success (P < .01). The chip budding method resulted in the maximum callus formation (36.7%), and graft success (30%). The rate of callus formation and graft success in the cleft grafting with semi-hard shoots was 2% and 18%, respectively. No callus formation and graft success were found in the cleft in hard stem and omega grafting methods. The graft success was zero in T-budding, despite of 18.33% callus formation. The results revealed the success of grafting of ‘Bidaneh’ on wild barberry species for the first time. However, increasing the graft success rate requires further research.

摘要

无核小檗“Bidaneh”是伊朗最重要的栽培小水果之一。该品种仅靠吸盘繁殖，短期内无法建立大规模果园。在这项研究中，评估了不同嫁接方法对'Bidaneh'在野生型小檗上的嫁接成功率。连续两年（2018年和2019年）进行了包括裂口嫁接（硬芽和绿/半硬芽）、欧米茄嫁接、T型芽接和芯片芽接（春中夏末）等1000多个嫁接方法）。结果表明，嫁接方式对愈伤组织形成率和嫁接成功率有显着影响（P< .01)。芯片出芽方法导致最大的愈伤组织形成 (36.7%) 和移植成功 (30%)。半硬枝裂接法愈伤组织形成率和嫁接成功率分别为2%和18%。在硬茎和欧米茄嫁接方法的裂缝中没有发现愈伤组织形成和嫁接成功。尽管有 18.33% 的愈伤组织形成，但 T 型出芽的移植成功率为零。结果表明，'Bidaneh' 首次成功嫁接到野生小檗品种上。然而，提高移植成功率需要进一步研究。