Aim: There are many people with type 2 Diabetes Mellitus (T2DM) worldwide. Rouxen- Y Gastric Bypass (RYGB) is an effective surgery for treating T2DM with beneficial effects on β cell metabolism. However, the mechanism of how RYGB affects the pancreas, is not clear. We focused on metabolic changes in the pancreas of rats following RYGBand to investigate complex postoperative pancreatic metabolic reprogramming and to understand how RYGB improves islet function.

Methods: We performed RYGBonstreptozotocin-induced male T2DM rats. Then we measured indicators such as weight, fasting GLU, etc. After 10 weeks, pancreatic tissues were removed to identify and quantify differentially expressed proteins. Furthermore, functional analysis of these proteins and their associated pathways was conducted by bioinformatics methods.

Results: After surgery, 451 differentially expressed proteins associated with the mechanism ofRYGB were identified. Protein levels of regenerating islet-derived protein 3 (Reg3A), cell division cycle-associated protein 2 (CDCA2), and ADP-ribosylation factor 1 (Arf1) varied greatly, and Arf1 may be a point target in diabetes.

Conclusion: This research shows that RYGB could treat T2DM through changes in the pancreatic proteins. It will give some new insight into the molecular mechanism behind the effect of RYGB.

目标：全世界有很多人患有 2 型糖尿病 (T2DM)。Rouxen-Y 胃绕道术 (RYGB) 是一种治疗 T2DM 的有效手术，对 β 细胞代谢具有有益影响。然而，RYGB 如何影响胰腺的机制尚不清楚。我们专注于 RYGBand 后大鼠胰腺的代谢变化，以研究复杂的术后胰腺代谢重编程，并了解 RYGB 如何改善胰岛功能。

方法：我们对 RYGB 链脲佐菌素诱导的雄性 T2DM 大鼠进行了实验。然后我们测量了体重、空腹 GLU 等指标。10 周后，取出胰腺组织以鉴定和量化差异表达的蛋白质。此外，这些蛋白质及其相关通路的功能分析是通过生物信息学方法进行的。

结果：术后共鉴定出451个与RYGB机制相关的差异表达蛋白。再生胰岛衍生蛋白 3 (Reg3A)、细胞分裂周期相关蛋白 2 (CDCA2) 和 ADP-核糖基化因子 1 (Arf1) 的蛋白质水平差异很大，Arf1 可能是糖尿病的一个点靶点。

结论：这项研究表明 RYGB 可以通过胰腺蛋白质的变化来治疗 T2DM。它将为 RYGB 效应背后的分子机制提供一些新的见解。