Molecular virology tools are critical for basic studies of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) and for developing new therapeutics. There remains a need for experimental systems that do not rely on viruses capable of spread that could potentially be used in lower containment settings. Here, we develop spike-deleted SARS-CoV-2 self-replicating RNAs using a yeast-based reverse genetics system. These non-infectious self-replicating RNAs, or replicons, can be trans-complemented with viral glycoproteins to generate Replicon Delivery Particles (RDPs) for single-cycle delivery into a range of cell types. This SARS-CoV-2 replicon system represents a convenient and versatile platform for antiviral drug screening, neutralization assays, host factor validation, and characterizing viral variants.

中文翻译：

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SARS-CoV-2 复制子的复制和单周期递送


分子病毒学工具对于严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 的基础研究和开发新疗法至关重要。仍然需要不依赖能够传播的病毒的实验系统，这些系统有可能在较低的遏制环境中使用。在这里，我们使用基于酵母的反向遗传学系统开发了尖峰删除的 SARS-CoV-2 自我复制 RNA。这些非感染性自我复制 RNA 或复制子可以与病毒糖蛋白反式互补，生成复制子递送颗粒 (RDP)，用于单周期递送到一系列细胞类型中。该 SARS-CoV-2 复制子系统为抗病毒药物筛选、中和测定、宿主因子验证和病毒变异表征提供了一个方便且多功能的平台。