当前位置: X-MOL 学术Science › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Replication and single-cycle delivery of SARS-CoV-2 replicons
Science ( IF 44.7 ) Pub Date : 2021-10-14 , DOI: 10.1126/science.abj8430
Inna Ricardo-Lax 1 , Joseph M Luna 1 , Tran Thi Nhu Thao 2, 3, 4 , Jérémie Le Pen 1 , Yingpu Yu 1 , H-Heinrich Hoffmann 1 , William M Schneider 1 , Brandon S Razooky 1 , Javier Fernandez-Martinez 5 , Fabian Schmidt 6 , Yiska Weisblum 6 , Bettina Salome Trüeb 2, 3 , Inês Berenguer Veiga 2, 3 , Kimberly Schmied 2, 3 , Nadine Ebert 2, 3 , Eleftherios Michailidis 1 , Avery Peace 1 , Francisco J Sánchez-Rivera 7 , Scott W Lowe 7 , Michael P Rout 5 , Theodora Hatziioannou 6 , Paul D Bieniasz 6 , John T Poirier 8 , Margaret R MacDonald 1 , Volker Thiel 2, 3 , Charles M Rice 1
Affiliation  

Molecular virology tools are critical for basic studies of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) and for developing new therapeutics. There remains a need for experimental systems that do not rely on viruses capable of spread that could potentially be used in lower containment settings. Here, we develop spike-deleted SARS-CoV-2 self-replicating RNAs using a yeast-based reverse genetics system. These non-infectious self-replicating RNAs, or replicons, can be trans-complemented with viral glycoproteins to generate Replicon Delivery Particles (RDPs) for single-cycle delivery into a range of cell types. This SARS-CoV-2 replicon system represents a convenient and versatile platform for antiviral drug screening, neutralization assays, host factor validation, and characterizing viral variants.

中文翻译:


SARS-CoV-2 复制子的复制和单周期递送



分子病毒学工具对于严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 的基础研究和开发新疗法至关重要。仍然需要不依赖能够传播的病毒的实验系统,这些系统有可能在较低的遏制环境中使用。在这里,我们使用基于酵母的反向遗传学系统开发了尖峰删除的 SARS-CoV-2 自我复制 RNA。这些非感染性自我复制 RNA 或复制子可以与病毒糖蛋白反式互补,生成复制子递送颗粒 (RDP),用于单周期递送到一系列细胞类型中。该 SARS-CoV-2 复制子系统为抗病毒药物筛选、中和测定、宿主因子验证和病毒变异表征提供了一个方便且多功能的平台。
更新日期:2021-10-15
down
wechat
bug