A label-free immunosensor using thin film transistor microelectrode arrays (TFT-EAs) derived from flat-panel technology has been developed for the first time. The TFT-EAs used here incorporate a large sensing area of 15 mm 15 mm with a 150 150 array of transparent indium tin oxide (ITO) electrodes placed at a 100 μm pixel pitch. Anti-human serum albumin (HSA) IgG was characterized to be immobilized on functionalized ITO electrodes. HSA, which is one of the indexes for liver functions, was successfully detected by recording the change of real-time current based on the change in the electric double layer capacitance before and after conjugation of HSA with anti-HSA IgG. Ionic strength and pH value of HSA solution were optimized. In 0.01 mM phosphate buffered saline with pH 6.0, linear range of HSA was determined as 0.3–300 pM. The present TFT-EAs are applicable to a label-free multiple immunosensing by patterning antibodies on its surface.

使用薄膜晶体管微电极阵列 (TFT-EA）首次开发出源自平板技术。TFT-此处使用的 EA 包含一个 15 mm × 15 mm 的大传感区域和一个 150 150 阵列的透明氧化铟锡 (ITO) 电极，像素间距为 100 μm。抗人血清白蛋白 (HSA) IgG 被表征为固定在功能化的 ITO 电极上。基于HSA与抗HSA IgG结合前后双电层电容的变化，通过记录实时电流的变化，成功检测出HSA是肝功能指标之一。优化了HSA溶液的离子强度和pH值。在 pH 6.0 的 0.01 mM 磷酸盐缓冲盐水中，HSA 的线性范围确定为 0.3-300 pM。目前的TFT-通过在其表面形成抗体图案，EA 适用于无标记的多重免疫传感。