Microelectrode arrays (MEAs) are crucial tools for evaluating the modulating effects during in vivo neuromodualtion and it is crucial to develop novel strategies that allow for high spatiotemporal resolution, sustainable potential and dynamic monitoring during neurological disease therapy. Herein, we used a hydrogelator to assemble with ruthenium-based light-sensitive compound, RuBi-GABA, to prepare a light-responsive supramolecular hydrogel for suppressing seizures in the cortex of epileptic rats, which benefited the prolonged release of RuBi-GABA. Meanwhile, the real-time variations in neuronal action potentials (Spikes) and local field potentials (LFPs) during the modulation were recorded by nanomaterial-modified implanted MEAs. The results demonstrated the mean firing rate and LFP power sharply decreased 54.95% and 76.56% due to the light-activation of RuBi-GABA hydrogel in a timely manner. Additionally, the high synchronicity between pyramidal neurons and interneurons efficiently decreased after the illumination. Furthermore, simultaneous modulation of spikes and LFPs in time and frequency domain demonstrated that cortical seizures were efficiently inhibited from microscopic to mesoscopic signals. We anticipated that the strategy of using RuBi-GABA hydrogel for seizure control with the simultaneous effectiveness evaluation by the MEAs could lead to a novel approach for epilepsy therapy.

微电极阵列 (MEA) 是评估体内调节作用的重要工具神经调节，开发新的策略以在神经疾病治疗期间实现高时空分辨率、可持续潜力和动态监测至关重要。在此，我们使用水凝胶剂与基于钌的光敏化合物 RuBi-GABA 组装，制备了一种光响应超分子水凝胶，用于抑制癫痫大鼠皮层的癫痫发作，这有利于 RuBi-GABA 的延长释放。同时，通过纳米材料修饰的植入 MEA 记录了调制过程中神经元动作电位 (Spikes) 和局部场电位 (LFP) 的实时变化。结果表明，由于 RuBi-GABA 水凝胶的光活化及时，平均放电率和 LFP 功率急剧下降了 54.95% 和 76.56%。此外，光照后锥体神经元和中间神经元之间的高度同步性有效降低。此外，在时域和频域中同时调制尖峰和 LFP 表明皮层癫痫发作从微观信号到细观信号被有效抑制。我们预计，使用 RuBi-GABA 水凝胶控制癫痫发作并同时通过 MEA 进行有效性评估的策略可能会导致癫痫治疗的新方法。