Utilizing tobacco industrial waste can increase added value without increasing the environmental burden. The physicochemical, structural, and functional properties of tobacco leaf powders produced by coarse and superfine grinding treatments with different particle size were investigated. The particle size was significantly reduced by superfine grinding to micron scale (10.3 μm), and the specific surface area sharply increased. With decreasing particle size, the bulk density, tap density, angles of repose and slide increased, but water and oil holding capacities decreased, indicating the worse flowability and hydration property. The increasing lightness and yellowness but decreasing redness were observed after superfine grinding. Differential scanning calorimetry curves showed that superfine grinding insignificantly affected the conformation stability and denaturation degree of proteins. Fourier transform infrared spectroscopy proved unchanged main structure and decreased chain length of polysaccharide polymer. Morphology revealed that the shape of superfine ground sample was changed from an irregular polygon to spherical-like, and surface roughness was reduced. All cell walls were disrupted after superfine grinding, but the dissolution yields of nicotine and total phenolic, and antioxidant activities were not enhanced. The median particle size of 93.9 μm was the most effective for the ingredient dissolution and antioxidant capacity. This study was expected to supply new insights in guiding the development of discarded tobacco in practice.

利用烟草工业废物可以在不增加环境负担的情况下增加附加值。研究了粗磨和超细磨不同粒径烟叶粉的理化、结构和功能特性。通过超细研磨，粒径显着降低至微米级（10.3 μm），比表面积急剧增加。随着粒径的减小，堆积密度、振实密度、休止角和滑动角增大，但保水持油能力下降，流动性和水化性变差。在超细研磨后观察到亮度和黄色增加但红色减少。差示扫描量热曲线表明，超细研磨对蛋白质的构象稳定性和变性程度影响不显着。傅里叶变换红外光谱证明多糖聚合物的主要结构不变，链长减少。形态学表明，超细研磨样品的形状由不规则多边形变为球形，表面粗糙度降低。超细研磨后所有细胞壁均被破坏，但尼古丁和总酚的溶解产率和抗氧化活性没有提高。93.9 μm 的中值粒径对成分溶解和抗氧化能力最有效。这项研究有望为指导废弃烟草的实践发展提供新的见解。