In this work, effects of sulfomethylated lignins (SLs) prepared from masson pine (SL_M) and poplar (SL_P) on enzymatic hydrolysis and cellulase-lignin interaction were comparatively investigated. The results showed that both SL_M and SL_P significantly promoted the substrate enzymatic digestibility. The total sugar yield increased from 38.6% to 74.4% and ∼ 100%, respectively at 10 FPU/g-cellulose of cellulase dosage. The protein content in hydrolysate linearly increased with the addition of SL (0 – 1.6 g/g-substrate lignin), which suggested the competitive adsorption of cellulase may occur to substrate lignin and SLs. Further structural analysis of lignin revealed the high S/(V + H) ratio was directly related to the high enzymatic saccharification efficiency. The strong interaction between SL and cellulase decreased the nonproductive adsorption of cellulase onto substrate lignin and increased the accessibility of cellulase to carbohydrate, which was considered to be the key factor for the improvement of substrate enzymatic digestibility.

在这项工作中，比较研究了从马尾松 (SL _M ) 和杨树 (S _P )制备的磺甲基化木质素 (SLs ) 对酶水解和纤维素酶-木质素相互作用的影响。结果表明，SL _M和 SL _P显着提高了底物的酶消化率。在纤维素酶剂量为 10 FPU/g-纤维素时，总糖产量分别从 38.6% 增加到 74.4% 和 ~ 100%。水解产物中的蛋白质含量随着SL的加入（0-1.6 g/g-底物木质素）而线性增加，这表明纤维素酶可能对底物木质素和SLs发生竞争吸附。木质素的进一步结构分析表明，高 S/(V + H) 比与高酶糖化效率直接相关。SL与纤维素酶之间的强相互作用减少了纤维素酶对底物木质素的非生产性吸附，增加了纤维素酶对碳水化合物的可及性，这被认为是提高底物酶消化率的关键因素。