Ocimum viride is known for its diverse medicinal uses. However, most previous studies have been conducted on phytochemical assessment, but intra-specific chemical variations are rarely reported. Chemical variations among seven genotypes of O. viride were studied over three consecutive years in the experimental farm in CSIR-CIMAP, Lucknow. The essential oils obtained by hydro-distillation from the aerial parts of O. viride genotypes were subjected to GC, enantiomeric GC, and GC/MS analyses for their chemical characterization. GC-FID analysis revealed maximum proportions of eugenol (37.8 %–45.8 %) along with monoterpenes such as (Z)-β-ocimene (9.3 %–29.5 %). The sesquiterpenoids identified were β-caryophyllene (3.1 %–4.6 %), α-trans-bergamotene (5.5 %–7.4 %) and germacrene D (5.9 %–10.6 %). Chiral separation of germacrene D enantiomers [(+)– and (−)–form] were achieved using a cyclodextrin-based capillary column in each genotype. (−)–Germacrene D was found to be the most predominant enantiomer in all genotypes. Genotype 133 was recorded with the highest enantiomeric excess (≥ 99 %) for (−)–germacrene D. Besides, genotype 131 contained high monoterpenoids. Major chemical constituents such as eugenol were isolated through column chromatography, and structure elucidation was carried out using 1D and 2D NMR experiments. The thermo-gravimetric analysis (TGA) showed the stability pattern of extracted essential oils and revealed the complete volatile degradation ranged from 160 °C–200 °C. The statistical analysis was performed to select the best genotypes. The study revealed the best genotypes based on the correlation between constituents × years (C × Y) interaction. These analyses helped better estimate essential oil yield analysis, chemical diversity, and herb yield analysis on a field experiment basis. In conclusion, genotypes 129 and 132 had the highest essential oil output and eugenol content (%). Eugenol, a marker chemical ingredient, was reported with the highest proportions in genotype 132 at the full bloom stage.

Ocimum viride以其多样化的药用用途而闻名。然而，以前的大多数研究都是对植物化学评估进行的，但很少报道种内化学变化。在勒克瑙 CSIR-CIMAP 的实验农场连续三年研究了七种O. viride基因型之间的化学变异。通过水蒸馏从O. viride基因型地上部分获得的精油进行 GC、对映体 GC 和 GC/MS 分析以进行化学表征。GC-FID 分析显示丁香酚 (37.8 %–45.8 %) 以及 ( Z )-β-罗勒烯 (9.3 %–29.5 %)等单萜的最大比例。鉴定的倍半萜类化合物为 β-石竹烯 (3.1 %–4.6 %)、α-反式-佛手柑 (5.5 %–7.4 %) 和锗烯 D (5.9 %–10.6 %)。使用基于环糊精的毛细管柱在每个基因型中实现了锗烯 D 对映异构体 [(+)– 和 (-)– 形式] 的手性分离。(-)-Germacrene D 被发现是所有基因型中最主要的对映异构体。基因型 133 的 (-)-germacrene D 对映体过量 (≥ 99 %) 记录到最高。此外，基因型 131 含有高单萜类化合物。通过柱色谱分离丁香酚等主要化学成分，并使用一维和二维核磁共振实验进行结构解析。热重分析 (TGA) 显示了提取精油的稳定性模式，并揭示了在 160 °C 至 200 °C 范围内的完全挥发性降解。进行统计分析以选择最佳基因型。该研究根据成分×年（C×Y）相互作用之间的相关性揭示了最佳基因型。这些分析有助于在田间试验的基础上更好地估计精油产量分析、化学多样性和药草产量分析。总之，基因型 129 和 132 具有最高的精油产量和丁香酚含量 (%)。丁香酚是一种标记化学成分，据报道在盛花期在基因型 132 中的比例最高。