Ruthenium red: a highly efficient and versatile cell staining agent for single-cell analysis using inductively coupled plasma time-of-flight mass spectrometry,Analyst

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Ruthenium red: a highly efficient and versatile cell staining agent for single-cell analysis using inductively coupled plasma time-of-flight mass spectrometry
Analyst ( IF 3.6 ) Pub Date : 2021-10-13 , DOI: 10.1039/d1an01143j
Wen Qin ₁ , Hans-Joachim Stärk ₁ , Thorsten Reemtsma _{1,

2}

Affiliation

Staining of biological cells with heavy metals can increase their visibility in mass spectrometry. In this study, the potential of ruthenium red (RR) as a staining agent for single-cell analysis by inductively coupled plasma time-of-flight mass spectrometry (SC-ICP-TOF-MS) is explored using two different yeast strains and one algal species. Time-of-flight mass spectrometry allows the simultaneous detection of Ru and multiple intrinsic elements in single cells. Ru has a better correlation with Mg than with P in Saccharomyces cerevisiae (S. cerevisiae) cells. For the three tested strains, the staining efficiency of RR exceeded 96%; the staining strengths were 30–32 ag μm⁻² for the yeast cells and 59 ag μm⁻² for the algal cells. By deriving the cell volume of single cells from their Ru mass, the concentration of Mg and P in individual cells of S. cerevisiae can be calculated. Elemental concentrations of Mg and P were highly variable in the cell individuals, with their 25–75 percentile values of 0.10–0.19 and 0.76–2.07 fg μm⁻³, respectively. RR staining has several advantages: it is fast, does not affect cell viability and is highly efficient. Provided that the shape of the individual cells of a culture is similar, Ru staining allows the elemental content to be directly correlated with the cell volume to accurately calculate the intracellular concentration of target elements in single cells. Therefore, RR can be a promising cell staining agent for future application in SC-ICP-TOF-MS research.

中文翻译：

钌红：一种高效且多功能的细胞染色剂，用于使用电感耦合等离子体飞行时间质谱法进行单细胞分析

用重金属对生物细胞进行染色可以提高它们在质谱分析中的可见度。在这项研究中，使用两种不同的酵母菌株和一种酵母菌株探索了钌红 (RR) 作为通过电感耦合等离子体飞行时间质谱 (SC-ICP-TOF-MS) 进行单细胞分析的染色剂的潜力。藻类。飞行时间质谱法允许同时检测单个细胞中的钌和多种内在元素。在酿酒酵母( S. cerevisiae ) 细胞中，Ru 与 Mg 的相关性优于与 P 的相关性。3株受试菌株，RR染色效率均超过96%；酵母细胞的染色强度为 30-32 ag μm ^{-2和 59 ag μm}^-2对于藻类细胞。通过从单个细胞的 Ru 质量推导单个细胞的细胞体积，可以计算出酿酒酵母单个细胞中 Mg 和 P 的浓度。Mg 和 P 的元素浓度在细胞个体中变化很大，它们的 25-75 百分位值为 0.10-0.19 和 0.76-2.07 fg μm ^-3，分别。RR 染色有几个优点：速度快、不影响细胞活力和高效。假设培养物中单个细胞的形状相似，Ru染色可以使元素含量与细胞体积直接相关，从而准确计算单个细胞中目标元素的细胞内浓度。因此，RR 可以成为未来在 SC-ICP-TOF-MS 研究中应用的有前途的细胞染色剂。

更新日期：2021-10-13

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