Regulation of the ThiM riboswitch is facilitated by the trapped structure formed during transcription of the wild-type sequence,FEBS Letters

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Regulation of the ThiM riboswitch is facilitated by the trapped structure formed during transcription of the wild-type sequence
FEBS Letters ( IF 3.0 ) Pub Date : 2021-10-13 , DOI: 10.1002/1873-3468.14202
Chengyi Du ₁ , Yujie Wang ₂ , Sha Gong ₁

Affiliation

The ThiM riboswitch from Escherichia coli is a typical mRNA device that modulates downstream gene expression by sensing TPP. The helix-based RNA folding theory is used to investigate its detailed regulatory behaviors in cells. This RNA molecule is transcriptionally trapped in a state with the unstructured SD sequence in the absence of TPP, which induces downstream gene expression. As a key step to turn on gene expression, formation of this trapped state (the genetic ON state) highly depends on the co-transcriptional folding of its wild-type sequence. Instead of stabilities of the genetic ON and OFF states, the transcription rate, pause, and ligand levels are combined to affect the ThiM riboswitch-mediated gene regulation, which is consistent with a kinetic control model.

中文翻译：

野生型序列转录过程中形成的被困结构促进了 ThiM 核糖开关的调节

来自大肠杆菌的ThiM核糖开关是一种典型的 mRNA 装置，它通过感知 TPP 来调节下游基因的表达。基于螺旋的 RNA 折叠理论用于研究其在细胞中的详细调节行为。在没有 TPP 的情况下，该 RNA 分子被转录捕获在具有非结构化 SD 序列的状态，从而诱导下游基因表达。作为开启基因表达的关键步骤，这种被困状态（遗传开启状态）的形成高度依赖于其野生型序列的共转录折叠。转录率、暂停和配体水平结合起来影响ThiM，而不是遗传 ON 和 OFF 状态的稳定性 核糖开关介导的基因调控，这与动力学控制模型一致。

更新日期：2021-11-22

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