Comprehensive Analysis of Mouse Hippocampal Lysine Acetylome Mediated by Sea Cucumber Peptides Preventing Memory Impairment,Journal of Agricultural and Food Chemistry

当前位置： X-MOL 学术 › J. Agric. Food Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Comprehensive Analysis of Mouse Hippocampal Lysine Acetylome Mediated by Sea Cucumber Peptides Preventing Memory Impairment
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-10-11 , DOI: 10.1021/acs.jafc.1c05155
Zhiqiang Lu ₁ , Xiaomeng Xu ₁ , Dongmei Li ₁ , Na Sun ₁ , Songyi Lin ₁

Affiliation

Memory impairment is becoming a potential health issue with the delicacy of diet and social stress. Sea cucumber peptides (SCP) prevent memory impairment, as previously reported. In this study, further research was performed using hippocampal lysine-acetylome to explore molecular regulation mechanisms. C57BL/6 mice were treated with scopolamine via intraperitoneal injection to simulate memory impairment. To determine the influence of SCP on the total acetylated-protein level of the hippocampus, acetylated-proteomics was performed. SCP increased the acetylation level of histone (H3 and H4). Meanwhile, for non-histones, the differentially acetylated proteins were involved in multiple memory-related pathways, as shown by KEGG enrichment analysis. Additionally, long-term potentiation was confirmed by western blotting. Finally, a combined analysis of proteome and lysine acetylome revealed that SCP contributed to synaptic vesicle cycle regulation and dopamine metabolism. Consequently, our findings revealed that SCP was potentially neuroprotective by regulating post-transcriptional hippocampal protein acetylation.

中文翻译：

海参肽介导小鼠海马赖氨酸乙酰化组预防记忆障碍的综合分析

记忆障碍正成为一个潜在的健康问题，因为饮食习惯和社会压力。如前所述，海参肽 (SCP) 可防止记忆障碍。本研究利用海马赖氨酸-乙酰基组进行进一步研究，探索分子调控机制。通过腹膜内注射用东莨菪碱治疗 C57BL/6 小鼠以模拟记忆障碍。为了确定 SCP 对海马总乙酰化蛋白质水平的影响，进行了乙酰化蛋白质组学。SCP 增加了组蛋白（H3 和 H4）的乙酰化水平。同时，对于非组蛋白，如 KEGG 富集分析所示，差异乙酰化蛋白参与多个记忆相关通路。此外，通过蛋白质印迹证实了长期增强。最后，对蛋白质组和赖氨酸乙酰组的综合分析表明，SCP 有助于突触囊泡循环调节和多巴胺代谢。因此，我们的研究结果表明，SCP 通过调节转录后海马蛋白乙酰化具有潜在的神经保护作用。

更新日期：2021-10-20

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11