Background

Osteosarcoma is the most common bone cancer that significantly affects the quality of life of patients. Studies have shown that overexpression of BAIAP2L2 elevates the proliferation and growth of some types of cancer cells. However, the role of BAIAP2L2 in osteosarcoma is unclear. This study aimed to investigate the functions of BAIAP2L2 in the development of osteosarcoma.

Methods

We used immunohistochemical and Western blot analysis to determine the expression levels of endogenic BAIAP2L2 in osteosarcoma cells. Cell counting kit-8 assay and colony formation assay were performed to investigate cell proliferation of tumor cells. Transwell assay was performed to detect cell migration. Flow cytometry assay was used to analyze cell apoptosis. The role of BAIAP2L2 in tumor growth was further explored in vivo.

Results

We found that BAIAP2L2 was significantly upregulated in human osteosarcoma, and inhibition of BAIAP2L2 suppressed the proliferation of osteosarcoma cells. In addition, down-regulation of BAIAP2L2 could lead to osteosarcoma cancer cell apoptosis, inhibit cell migration and invasion, and induce the inactivation of the Wnt/β-catenin pathway. In addition, down-regulation of BAIAP2L2 inhibited tumor growth in vivo.

Conclusion

In conclusion, down-regulation of BAIAP2L2 inhibited the proliferation, migration, and invasion of osteosarcoma associated with the Wnt/β-catenin pathway.

中文翻译：

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BAIAP2L2通过Wnt/β-catenin通路促进骨肉瘤的增殖、迁移和侵袭

 背景

骨肉瘤是最常见的骨癌，严重影响患者的生活质量。研究表明，BAIAP2L2 的过度表达会促进某些类型癌细胞的增殖和生长。然而，BAIAP2L2 在骨肉瘤中的作用尚不清楚。本研究旨在探讨BAIAP2L2在骨肉瘤发生发展中的功能。

 方法

我们使用免疫组织化学和蛋白质印迹分析来确定骨肉瘤细胞中内源性 BAIAP2L2 的表达水平。进行细胞计数试剂盒8测定和集落形成测定来研究肿瘤细胞的细胞增殖。进行Transwell实验来检测细胞迁移。采用流式细胞术分析细胞凋亡。在体内进一步探讨了BAIAP2L2在肿瘤生长中的作用。

 结果

我们发现 BAIAP2L2 在人骨肉瘤中显着上调，抑制 BAIAP2L2 可以抑制骨肉瘤细胞的增殖。此外，BAIAP2L2的下调可导致骨肉瘤癌细胞凋亡，抑制细胞迁移和侵袭，并诱导Wnt/β-catenin通路失活。此外，BAIAP2L2的下调抑制了体内肿瘤的生长。

 结论

总之，BAIAP2L2的下调抑制了与Wnt/β-catenin通路相关的骨肉瘤的增殖、迁移和侵袭。