The present research endeavour was executed with 59 rye introgressed wheat lines including 52 triticale × wheat stable lines and 7 wheat × rye (Lahaul Local) introgressed doubled haploids. The detection of new recombinants through molecular cytogenetic tools viz. genomic in situ hybridization and fluorescence in situ hybridization revealed 1BL.1RS translocation in 33 lines, 1BL.1RS translocation along with substitution in 5 lines, 1BL.1RS translocation along with addition in 8 lines, substitution of single pair of rye chromosomes in 5 lines and addition of rye chromatin in 1 line, while 7 lines were found with no rye chromatin. To validate the effect of rye introgression on drought tolerance and yellow rust resistance, all 59 recombinants were evaluated under well-watered and water stressed conditions at in vivo. Analysis of variance revealed sufficient genetic variability among genotypes for different traits at both in vivo level. Under field conditions, direct influence of rye introgression was observed on plant height, grain yield, biological yield and harvest index. Overall, 1BL.1RS translocations and substitutions were found to exhibit significant influence on most of the drought parameters. The screening for yellow rust resistance revealed differential disease reaction, where 52 recombinants were observed to be resistant. The lines exhibiting superiority for the traits can be used as a parental source in wheat improvement endeavours.

目前的研究工作是对 59 个黑麦基因渗入的小麦品系进行的，其中包括 52 个小黑麦 × 小麦稳定品系和 7 个小麦 × 黑麦（Lahaul Local）基因渗入的双单倍体。通过分子细胞遗传学工具检测新的重组体，即。基因组原位杂交和荧光原位杂交显示33个品系1BL.1RS易位，5个品系1BL.1RS易位伴随置换，8个品系1BL.1RS易位伴随添加，5个品系单对黑麦染色体置换1行加入黑麦染色质，7行无黑麦染色质。为了验证黑麦基因渗入对耐旱性和抗黄锈病的影响，在体内水分充足和水分胁迫的条件下对所有 59 种重组体进行了评估。方差分析表明，在体内水平上，不同性状的基因型之间存在足够的遗传变异性。在田间条件下，观察到黑麦基因渗入对株高、籽粒产量、生物产量和收获指数的直接影响。总体来说，1BL。发现 1RS 易位和取代对大多数干旱参数有显着影响。黄锈病抗性筛选揭示了差异疾病反应，其中观察到52个重组体具有抗性。在性状方面表现出优势的品系可用作小麦改良努力中的亲本来源。