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Dual-targeting SERS-encoded graphene oxide nanocarrier for intracellular co-delivery of doxorubicin and 9-aminoacridine with enhanced combination therapy
Analyst ( IF 3.6 ) Pub Date : 2021-09-27 , DOI: 10.1039/d1an01237a Hui Chen 1 , Longqiang Xing 1 , Huiru Guo 1 , Caixia Luo 1 , Xuedian Zhang 1
Analyst ( IF 3.6 ) Pub Date : 2021-09-27 , DOI: 10.1039/d1an01237a Hui Chen 1 , Longqiang Xing 1 , Huiru Guo 1 , Caixia Luo 1 , Xuedian Zhang 1
Affiliation
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A graphene oxide (GO)-based nanocarrier that imparts tumor-selective delivery of dual-drug with enhanced therapeutic index, is introduced. GO is conjugated with Au@Ag and Fe3O4 nanoparticles, which facilitates it with SERS tracking and magnetic targeting abilities, followed by the covalent binding of the anti-HER2 antibody, thus allowing it to both actively and passively target SKBR3 cells, human breast cancer cells expressed with HER2. Intracellular drug delivery behaviors are probed using SERS spectroscopy in a spatiotemporal manner, which demonstrates that nanocarriers are internalized into the lysosomes and release the drug in response to the acidic microenvironment. The nanocarriers loaded with dual-drug possess increased cancer cytotoxicity in comparison to those loaded with a single drug. Attractively, the enhanced cytotoxicity against cancer cells is achieved with relatively low concentrations of the drug, which is demonstrated to be involved in the drug adsorption status. These results may give us the new prospects to design GO-based delivery systems with rational drug dosages, thus achieving optimal therapeutic response of the multi-drug with increased tumor selectivity and reduced side effects.
中文翻译：
双靶向 SERS 编码的氧化石墨烯纳米载体用于多柔比星和 9-氨基吖啶的细胞内共递送以及增强的联合治疗
介绍了一种基于氧化石墨烯 (GO) 的纳米载体，该载体可提供具有增强治疗指数的双药肿瘤选择性递送。GO 与 Au@Ag 和 Fe 3 O 4共轭纳米粒子，这有助于它具有 SERS 跟踪和磁性靶向能力，然后是抗 HER2 抗体的共价结合，从而使其能够主动和被动地靶向 SKBR3 细胞，即用 HER2 表达的人类乳腺癌细胞。使用 SERS 光谱以时空方式探测细胞内药物递送行为，这表明纳米载体被内化到溶酶体中并响应酸性微环境释放药物。与负载单一药物的纳米载体相比，负载双药物的纳米载体具有更高的癌症细胞毒性。有吸引力的是，通过相对低浓度的药物实现了对癌细胞的增强细胞毒性，这被证明与药物吸附状态有关。
更新日期：2021-10-12
中文翻译：
双靶向 SERS 编码的氧化石墨烯纳米载体用于多柔比星和 9-氨基吖啶的细胞内共递送以及增强的联合治疗
介绍了一种基于氧化石墨烯 (GO) 的纳米载体，该载体可提供具有增强治疗指数的双药肿瘤选择性递送。GO 与 Au@Ag 和 Fe 3 O 4共轭纳米粒子，这有助于它具有 SERS 跟踪和磁性靶向能力，然后是抗 HER2 抗体的共价结合，从而使其能够主动和被动地靶向 SKBR3 细胞，即用 HER2 表达的人类乳腺癌细胞。使用 SERS 光谱以时空方式探测细胞内药物递送行为，这表明纳米载体被内化到溶酶体中并响应酸性微环境释放药物。与负载单一药物的纳米载体相比，负载双药物的纳米载体具有更高的癌症细胞毒性。有吸引力的是，通过相对低浓度的药物实现了对癌细胞的增强细胞毒性，这被证明与药物吸附状态有关。
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