Background Rotavirus is a leading cause of pediatric diarrheal mortality. The rotavirus outer capsid consists of VP7 and VP4 proteins, which, respectively, determine viral G and P type and are primary targets of neutralizing antibodies. Methods To elucidate VP7-specific neutralizing antibody responses, we engineered monoreassortant rotaviruses each containing a human VP7 segment from a sequenced clinical specimen or a vaccine strain in an identical genetic background. We quantified replication and neutralization of engineered viruses using sera from infants vaccinated with monovalent ROTARIX or multivalent RotaTeq vaccines. Results Immunization with RotaTeq induced broader neutralizing antibody responses than ROTARIX. Inclusion of a single dose of RotaTeq in the schedule enhanced G-type neutralization breadth of vaccinated infant sera. Cell type-specific differences in infectivity, replication, and neutralization were detected for some monoreassortant viruses. Conclusions These findings suggest that rotavirus VP7, independent of VP4, can contribute to cell tropism and the breadth of vaccine-elicited neutralizing antibody responses.

中文翻译：

---

接种 ROTARIX 和 RotaTeq 疫苗的婴儿血清可差异中和多种 G 型的单重排轮状病毒

背景 轮状病毒是儿童腹泻死亡的主要原因。轮状病毒外衣壳由 VP7 和 VP4 蛋白组成，分别决定病毒 G 型和 P 型，是中和抗体的主要靶标。方法 为了阐明 VP7 特异性中和抗体反应，我们设计了单重排轮状病毒，每个轮状病毒都包含来自相同遗传背景的已测序临床标本或疫苗株的人类 VP7 片段。我们使用接种了单价 ROTARIX 或多价 RotaTeq 疫苗的婴儿血清对工程病毒的复制和中和进行了量化。结果 与 ROTARIX 相比，RotaTeq 免疫诱导更广泛的中和抗体反应。在时间表中加入单剂量 RotaTeq 增强了接种婴儿血清的 G 型中和广度。检测到一些单重组病毒在感染性、复制和中和方面的细胞类型特异性差异。结论 这些发现表明，独立于 VP4 的轮状病毒 VP7 可以促进细胞向性和疫苗引发的中和抗体反应的广度。