The success of establishing bioeconomies replacing current economies based on fossil resources largely depends on our ability to degrade recalcitrant lignocellulosic biomass. This study explores the potential of employing various enzymes acting synergistically on previously pretreated agricultural side streams (corn bran, oat hull, soluble and insoluble oat bran). Degrees of synergy (oligosaccharide yield obtained with the enzyme combination divided by the sum of yields obtained with individual enzymes) of up to 88 were obtained. Combinations of a ferulic acid esterase and xylanases resulted in synergy on all substrates, while a laccase and xylanases only acted synergistically on the more recalcitrant substrates. Synergy between different xylanases (glycoside hydrolase (GH) families 5 and 11) was observed particularly on oat hulls, producing a yield of 57%. The synergistic ability of the enzymes was found to be partly due to the increased enzyme stability when in combination with the substrates.

建立生物经济以取代当前基于化石资源的经济的成功很大程度上取决于我们降解顽固的木质纤维素生物质的能力。本研究探讨了使用各种酶协同作用于先前预处理的农业侧流（玉米麸皮、燕麦壳、可溶性和不溶性燕麦麸皮）的潜力。获得了高达 88 的协同作用程度（用酶组合获得的寡糖产量除以用单个酶获得的产量总和）。阿魏酸酯酶和木聚糖酶的组合导致对所有底物的协同作用，而漆酶和木聚糖酶仅协同作用于更顽固的底物。特别是在燕麦壳上观察到不同木聚糖酶（糖苷水解酶 (GH) 家族 5 和 11）之间的协同作用，产率为 57%。发现酶的协同能力部分是由于与底物组合时酶稳定性增加。