Dioscorea zingiberensis is a medicinal herb containing a large amount of steroidal saponins, which are the major bioactive compounds and the primary storage form of diosgenin. The CYP72A gene family, belonging to cytochromes P450, exerts indispensable effects on the biosynthesis of numerous bioactive compounds. In this work, a total of 25 CYP72A genes were identified in D. zingiberensis and categorized into two groups according to the homology of protein sequences. The characteristics of their phylogenetic relationship, intron–exon organization, conserved motifs and cis-regulatory elements were performed by bioinformatics methods. The transcriptome data demonstrated that expression patterns of DzCYP72As varied by tissues. Moreover, qRT-PCR results displayed diverse expression profiles of DzCYP72As under different concentrations of jasmonic acid (JA). Likewise, eight metabolites in the biosynthesis pathway of steroidal saponins (four phytosterols, diosgenin, parvifloside, protodeltonin and dioscin) exhibited different contents under different concentrations of JA, and the content of total steroidal saponin was largest at the dose of 100 μmol/L of JA. The redundant analysis showed that 12 DzCYP72As had a strong correlation with specialized metabolites. Those genes were negatively correlated with stigmasterol and cholesterol but positively correlated with six other specialized metabolites. Among all DzCYP72As evaluated, DzCYP72A6, DzCYP72A16 and DzCYP72A17 contributed the most to the variation of specialized metabolites in the biosynthesis pathway of steroidal saponins. This study provides valuable information for further research on the biological functions related to steroidal saponin biosynthesis.

中文翻译：

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CYP72A 基因家族的全基因组鉴定以及与茉莉酸处理和姜黄中甾体皂苷积累相关的表达模式

薯蓣是一种药用植物，含有大量甾体皂苷，是主要的生物活性化合物和薯蓣皂苷元的主要储存形式。该CYP72A基因家族，属于细胞色素P450，发挥在许多生物活性化合物的生物合成必不可少的作用。在这项工作中，总共在D中鉴定了 25 个CYP72A基因。zingiberensis并根据蛋白质序列的同源性分为两类。它们的系统发育关系、内含子-外显子组织、保守基序和顺式调控元件的特征通过生物信息学方法进行。转录组数据表明，DzCYP72As因组织而异。此外，qRT-PCR 结果显示了不同浓度茉莉酸 (JA) 下DzCYP72As 的不同表达谱。同样，甾体皂苷生物合成途径中的8种代谢物（四种植物甾醇、薯蓣皂苷、小叶皂甙、原deltonin和薯蓣皂甙）在不同浓度的JA下表现出不同的含量，其中总甾体皂苷的含量在100 μmol/L的剂量下最大。 JA。冗余分析表明，12 个DzCYP72A与特化代谢物有很强的相关性。这些基因与豆甾醇和胆固醇呈负相关，但与其他六种特殊代谢物呈正相关。在评估的所有DzCYP72As 中，DzCYP72A6、DzCYP72A16和DzCYP72A17对甾体皂苷生物合成途径中特化代谢物的变化贡献最大。该研究为进一步研究甾体皂苷生物合成相关的生物学功能提供了有价值的信息。