In this paper, we report the in situ growth of FF nanotubes examined via liquid-cell transmission electron microscopy (LCTEM). This direct, high spatial, and temporal resolution imaging approach allowed us to observe the growth of peptide-based nanofibrillar structures through directional elongation. Furthermore, the radial growth profile of FF nanotubes through the addition of monomers perpendicular to the tube axis has been observed in real-time with sufficient resolution to directly observe the increase in diameter. Our study demonstrates that the kinetics, dynamics, structure formation, and assembly mechanism of these supramolecular assemblies can be directly monitored using LCTEM. The performance of the peptides and the assemblies they form can be verified and evaluated using post-mortem techniques including time-of-flight secondary ion mass spectrometry (ToF-SIMS).

中文翻译：

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二肽纳米结构组装和动力学通过原位液相电子显微镜

在本文中，我们报告了通过液体细胞透射电子显微镜 (LCTEM)检查的 FF 纳米管的原位生长。这种直接、高空间和时间分辨率的成像方法使我们能够通过定向伸长观察基于肽的纳米纤维结构的生长。此外，通过添加垂直于管轴的单体，FF 纳米管的径向生长曲线已经实时观察到，分辨率足以直接观察直径的增加。我们的研究表明，可以使用 LCTEM 直接监测这些超分子组装体的动力学、动力学、结构形成和组装机制。可以使用以下方法验证和评估肽及其形成的组件的性能验尸技术，包括飞行时间二次离子质谱法 (ToF-SIMS)。