Trichoderma is a genus of filamentous fungi that play notable roles in stimulating plant growth after colonizing the root surface. However, the key proteins and molecular mechanisms governing this stimulation have not been completely elucidated. In this study, Trichoderma guizhouense NJAU 4742 was investigated in a hydroponic culture system after interacting with cucumber roots. The total proteins of the fungus were characterized, and the key metabolic pathways along with related genes were analyzed through proteomic and transcriptomic analyses. The roles played by the regulated proteins during the interaction between plants and NJAU 4742 were further examined. The intracellular or extracellular proteins from NJAU 4742 and extracellular proteins from cucumber were quantified, and the high-abundance proteins were determined which were primarily involved in the shikimate pathway (tryptophan, tyrosine, and phenylalanine metabolism, auxin biosynthesis, and secondary metabolite synthesis). Moreover, ¹⁵N-KNO₃ labeling analysis indicated that NJAU 4742 had a strong ability to convert nitrogenous amino acids, nitrate, nitrile, and amines into ammonia. The auxin synthesis and ammonification metabolism pathways of NJAU 4742 significantly contributed to plant growth. The results of this study demonstrated the crucial metabolic pathways involved in the interactions between Trichoderma spp. and plants.

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木霉属是一种丝状真菌，在定植根表面后在刺激植物生长方面发挥显着作用。然而，控制这种刺激的关键蛋白质和分子机制尚未完全阐明。本研究中，贵州木霉NJAU 4742 在与黄瓜根相互作用后在水培系统中进行了研究。表征了真菌的总蛋白质，并通过蛋白质组学和转录组学分析分析了关键代谢途径以及相关基因。进一步检查了受调控的蛋白质在植物与 NJAU 4742 之间的相互作用过程中所起的作用。对来自 NJAU 4742 的细胞内或细胞外蛋白质和来自黄瓜的细胞外蛋白质进行定量，并确定主要参与莽草酸途径（色氨酸、酪氨酸和苯丙氨酸代谢、生长素生物合成和次级代谢物合成）的高丰度蛋白质。此外，¹⁵ N-KNO ₃标记分析表明NJAU 4742具有很强的将含氮氨基酸、硝酸盐、腈和胺转化为氨的能力。NJAU 4742 的生长素合成和氨化代谢途径显着促进植物生长。这项研究的结果证明了参与木霉属之间相互作用的关键代谢途径。和植物。

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