Direct SARS-CoV-2 Nucleic Acid Detection by Y-Shaped DNA Dual-Probe Transistor Assay,Journal of the American Chemical Society

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Direct SARS-CoV-2 Nucleic Acid Detection by Y-Shaped DNA Dual-Probe Transistor Assay
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2021-10-08 , DOI: 10.1021/jacs.1c06325
Derong Kong _{1,

2} , Xuejun Wang _{1,

2} , Chenjian Gu ₃ , Mingquan Guo ₄ , Yao Wang ₃ , Zhaolin Ai _{1,

2} , Shen Zhang _{1,

2} , Yiheng Chen _{1,

2} , Wentao Liu _{1,

2} , Yungen Wu _{1,

2} , Changhao Dai _{1,

2} , Qianying Guo _{1,

2} , Di Qu ₃ , Zhaoqin Zhu ₄ , Youhua Xie ₃ , Yunqi Liu _{2,

5} , Dacheng Wei _{1,

2}

Affiliation

Rapid screening of infected individuals from a large population is an effective means in epidemiology, especially to contain outbreaks such as COVID-19. The gold standard assays for COVID-19 diagnostics are mainly based on the reverse transcription polymerase chain reaction, which mismatches the requirements for wide-population screening due to time-consuming nucleic acid extraction and amplification procedures. Here, we report a direct nucleic acid assay by using a graphene field-effect transistor (g-FET) with Y-shaped DNA dual probes (Y-dual probes). The assay relies on Y-dual probes modified on g-FET simultaneously targeting ORF1ab and N genes of SARS-CoV-2 nucleic acid, enabling high a recognition ratio and a limit of detection (0.03 copy μL^–1) 1–2 orders of magnitude lower than existing nucleic acid assays. The assay realizes the fastest nucleic acid testing (∼1 min) and achieves direct 5-in-1 pooled testing for the first time. Owing to its rapid, ultrasensitive, easily operated features as well as capability in pooled testing, it holds great promise as a comprehensive tool for population-wide screening of COVID-19 and other epidemics.

中文翻译：

通过 Y 型 DNA 双探针晶体管检测直接检测 SARS-CoV-2 核酸

从大量人群中快速筛查感染者是流行病学中的一种有效手段，特别是在遏制 COVID-19 等疫情方面。COVID-19 诊断的金标准检测主要基于逆转录聚合酶链式反应，由于核酸提取和扩增程序耗时，因此不符合大范围人群筛查的要求。在这里，我们报告了使用带有 Y 形 DNA 双探针（Y-双探针）的石墨烯场效应晶体管（g-FET）进行的直接核酸测定。该检测依赖于 g-FET 修饰的 Y 双探针，同时靶向 SARS-CoV-2 核酸的 ORF1ab 和 N 基因，可实现高识别率和检测限 (0.03 copy µL –1 ) 1-2 个^数量级数量级低于现有的核酸检测。该检测实现了最快的核酸检测（约1分钟），并首次实现了直接五合一混合检测。由于其快速、超灵敏、易于操作的特点以及汇总测试的能力，它有望作为一种用于全人群筛查 COVID-19 和其他流行病的综合工具。

更新日期：2021-10-20

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