Potassium (K) is a major plant nutrient, and its deficiency can limit plant growth and development. Coronatine (COR) could increase cotton seedling tolerance to K deficiency, which was hypothesized to be in relation to improving the physiological and proteomic profile of xylem sap. To test this hypothesis, cotton seedlings growth, physio-biochemical and proteomic profile of xylem sap treated with (0 and 10 nM COR) under deficient K solution (0.05 mM KCl) were explored. Compared with control, COR treatment significantly increased lateral root number and root diameter and decreased contents of malondialdehyde (MDA), some cations like potassium and calcium in xylem sap and its volume. These morphological and physiological presentations were well evidenced by differentially expressed proteins (DEPs) in the xylem sap. For instance, increasing the average root diameter and lateral roots were related to up-regulation of cobalamin-independent methionine synthase family proteins, auxin-responsive protein, and cell wall remodeling proteins such as dirigent-like protein, laccase, and the pectin lyase; lessening xylem sap volume and some cations contents was in connections with down-regulation of uclacyanin 1 and up-regulation of calmodulin-domain protein kinase 7; the MDA content reducing was associated with many PCD-related proteins’ down-regulation or loss. Furthermore, COR potentially weakened plant defense, owing to lessening or disappearing lipid-transfer proteins, signaling proteins, and other proteins positively involved in plant defense.

钾（K）是一种主要的植物营养素，其缺乏会限制植物的生长发育。Coronatine (COR) 可以增加棉花幼苗对 K 缺乏的耐受性，这被假设与改善木质部汁液的生理和蛋白质组学特征有关。为了验证这一假设，研究了在缺钾溶液（0.05 mM KCl）下用（0 和 10 nM COR）处理的木质部汁液的棉花幼苗生长、生理生化和蛋白质组学特征。与对照相比，COR处理显着增加了侧根数和根径，降低了木质部汁液中丙二醛（MDA）、钾和钙等一些阳离子的含量及其体积。木质部汁液中的差异表达蛋白 (DEP) 很好地证明了这些形态学和生理学表现。例如，增加平均根直径和侧根与不依赖钴胺素的蛋氨酸合酶家族蛋白、生长素响应蛋白和细胞壁重塑蛋白（如 dirigent 样蛋白、漆酶和果胶裂解酶）的上调有关；木质部汁液体积和一些阳离子含量的减少与核蓝素 1 的下调和钙调蛋白域蛋白激酶 7 的上调有关；MDA 含量降低与许多 PCD 相关蛋白的下调或丢失有关。此外，由于脂质转移蛋白、信号蛋白和其他积极参与植物防御的蛋白质减少或消失，COR 可能会削弱植物防御。生长素反应蛋白和细胞壁重塑蛋白，如dirigent样蛋白、漆酶和果胶裂解酶；木质部汁液体积和一些阳离子含量的减少与核蓝素 1 的下调和钙调蛋白域蛋白激酶 7 的上调有关；MDA 含量降低与许多 PCD 相关蛋白的下调或丢失有关。此外，由于脂质转移蛋白、信号蛋白和其他积极参与植物防御的蛋白质减少或消失，COR 可能会削弱植物防御。生长素反应蛋白和细胞壁重塑蛋白，如dirigent样蛋白、漆酶和果胶裂解酶；木质部汁液体积和一些阳离子含量的减少与核蓝素 1 的下调和钙调蛋白域蛋白激酶 7 的上调有关；MDA 含量降低与许多 PCD 相关蛋白的下调或丢失有关。此外，由于脂质转移蛋白、信号蛋白和其他积极参与植物防御的蛋白质减少或消失，COR 可能会削弱植物防御。MDA 含量降低与许多 PCD 相关蛋白的下调或丢失有关。此外，由于脂质转移蛋白、信号蛋白和其他积极参与植物防御的蛋白质减少或消失，COR 可能会削弱植物防御。MDA 含量降低与许多 PCD 相关蛋白的下调或丢失有关。此外，由于脂质转移蛋白、信号蛋白和其他积极参与植物防御的蛋白质减少或消失，COR 可能会削弱植物防御。