DNA Tiling Enables Precise Acylation-Based Labeling and Control of mRNA,Angewandte Chemie International Edition

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DNA Tiling Enables Precise Acylation-Based Labeling and Control of mRNA
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2021-10-08 , DOI: 10.1002/anie.202112106
Lu Xiao ₁ , Yong Woong Jun ₁ , Eric T Kool ₁

Affiliation

A general method that enables the site-localized acylation of mRNA at virtually any determined sites is described. The TRAIL method utilizes a novel DNA tiling strategy by hybridizing a pool of inexpensive “protector” DNAs to block mRNA and combining with an “inducer” DNA to extrude a reactive RNA loop for acylation. This approach can be employed for both mRNA labeling and control for multiple applications.

中文翻译：

DNA 平铺可实现精确的基于酰化的 mRNA 标记和控制

描述了一种通用方法，该方法能够在几乎任何确定的位点对 mRNA 进行位点定位的酰化。TRAIL 方法利用一种新的 DNA 平铺策略，通过杂交一组廉价的“保护者”DNA 来阻断 mRNA，并与“诱导者”DNA 结合以挤出反应性 RNA 环以进行酰化。这种方法可用于 mRNA 标记和多种应用的控制。

更新日期：2021-12-06

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