A sequential cathode-anode cascade mode bioelectrochemical system (BES) was designed and developed to achieve the “self-degradation” of 2-chlorophenol (2-CP). With the cooperation of cathode and anode, the electrons supplied for the cathode 2-CP dechlorination come from its own dechlorinated product in the anode, phenol. Separate degradation experiments of cathode 2-CP and anode phenol were firstly conducted. The optimum concentration ratio of anode acetate to phenolic compound (3.66/1.56) and the phenolic compound degradation ability of BES were investigated. With the formation of the bioanode able to degrade phenol, the sequential cathode-anode cascade mode BES was further developed, where 2-CP could achieve sequential dechlorination and ring-cleavage degradation. When applied voltage was 0.6 V and cathode influent pH was 7, 1.56 mM 2-CP reached 80.15% cathode dechlorination efficiency and 58.91% total cathode-anode phenolic compounds degradation efficiency. The bioanodes played a decisive role in BES. Different operating conditions would affect the overall performance of BES by changing the electrochemical activity and microbial community structure of the bioanodes. This study demonstrated the feasibility of the sequential cathode-anode cascade mode BES to degrade 2-CP wastewater and provided perspectives for the cooperation of cathode and anode, aiming to explore more potential of BES in wastewater treatment field.

设计并开发了顺序阴极 - 阳极级联模式生物电化学系统（BES）以实现2-氯苯酚（2-CP）的“自降解”。在阴极和阳极的配合下，为阴极 2-CP 脱氯提供的电子来自其自身在阳极中的脱氯产物苯酚。首先进行了正极2-CP和负极苯酚的单独降解实验。考察了阳极乙酸盐与酚类化合物的最佳浓度比（3.66/1.56）和BES对酚类化合物的降解能力。随着能够降解苯酚的生物阳极的形成，进一步开发了顺序阴极 - 阳极级联模式 BES，其中 2-CP 可以实现顺序脱氯和开环降解。当施加的电压为 0.6 V 且阴极进水 pH 值为 7, 1 时。56 mM 2-CP 达到 80.15% 的阴极脱氯效率和 58.91% 的阴极-阳极酚类化合物总降解效率。生物阳极在 BES 中起到了决定性的作用。不同的操作条件会通过改变生物阳极的电化学活性和微生物群落结构来影响 BES 的整体性能。本研究证明了连续阴极-阳极级联模式BES降解2-CP废水的可行性，并为阴极和阳极的合作提供了前景，旨在探索BES在废水处理领域的更多潜力。不同的操作条件会通过改变生物阳极的电化学活性和微生物群落结构来影响 BES 的整体性能。本研究证明了连续阴极-阳极级联模式BES降解2-CP废水的可行性，并为阴极和阳极的合作提供了前景，旨在探索BES在废水处理领域的更多潜力。不同的操作条件会通过改变生物阳极的电化学活性和微生物群落结构来影响 BES 的整体性能。本研究证明了连续阴极-阳极级联模式BES降解2-CP废水的可行性，并为阴极和阳极的合作提供了前景，旨在探索BES在废水处理领域的更多潜力。