Thauera, as one of the core members of wastewater biological treatment systems, plays an important role in the process of nitrogen and phosphorus removal from low-carbon source sewage. However, there is a lack of systematic understanding of Thauera’s metabolic pathway and genomics. Here we report on the newly isolated Thauera sp. RT1901, which is capable of denitrification using variety carbon sources including aromatic compounds. By comparing the denitrification processes under the conditions of insufficient, adequate and surplus carbon sources, it was found that strain RT1901 could simultaneously use soluble microbial products (SMP) and extracellular polymeric substances (EPS) as electron donors for denitrification. Strain RT1901 was also found to be a denitrifying phosphate accumulating bacterium, able to use nitrate, nitrite, or oxygen as electron acceptors during poly-β-hydroxybutyrate (PHB) catabolism. The annotated genome was used to reconstruct the complete nitrogen and phosphorus metabolism pathways of RT1901. In the process of denitrifying phosphorus accumulation, glycolysis was the only pathway for glycogen metabolism, and the glyoxylic acid cycle replaced the tricarboxylic acid cycle (TCA) to supplement the reduced energy. In addition, the abundance of conventional phosphorus accumulating bacteria decreased significantly and the removal rates of total nitrogen (TN) and chemical oxygen demand (COD) increased after the addition of RT1901 in the low carbon/nitrogen (C/N) ratio of anaerobic aerobic anoxic-sequencing batch reactor (AOA-SBR). This research indicated that the diverse metabolic capabilities of Thauera made it more competitive than other bacteria in the wastewater treatment system.

Thauera作为废水生物处理系统的核心成员之一，在低碳源污水脱氮除磷过程中发挥着重要作用。然而，对Thauera的代谢途径和基因组学缺乏系统的了解。在这里，我们报告了新孤立的Thauerasp. RT1901，能够使用包括芳香族化合物在内的多种碳源进行反硝化。通过比较碳源不足、充足和过剩条件下的反硝化过程，发现菌株RT1901可以同时利用可溶性微生物产物（SMP）和胞外聚合物（EPS）作为电子供体进行反硝化。还发现菌株 RT1901 是一种反硝化磷酸盐积累细菌，能够在聚-β-羟基丁酸盐 (PHB) 分解代谢过程中使用硝酸盐、亚硝酸盐或氧作为电子受体。注释的基因组用于重建RT1901的完整氮和磷代谢途径。在反硝化磷的积累过程中，糖酵解是糖原代谢的唯一途径，乙醛酸循环取代了三羧酸循环（TCA）以补充减少的能量。此外，在厌氧好氧低碳氮（C/N）比中加入RT1901后，常规蓄磷菌丰度显着降低，总氮（TN）和化学需氧量（COD）去除率提高。缺氧序批式反应器 (AOA-SBR)。该研究表明，不同的代谢能力 在厌氧好氧缺氧测序低碳氮（C/N）比中加入RT1901后，常规蓄磷菌丰度显着降低，总氮（TN）和化学需氧量（COD）去除率提高间歇式反应器（AOA-SBR）。该研究表明，不同的代谢能力 在厌氧好氧缺氧测序低碳氮（C/N）比中加入RT1901后，常规蓄磷菌丰度显着降低，总氮（TN）和化学需氧量（COD）去除率提高间歇式反应器（AOA-SBR）。该研究表明，不同的代谢能力Thauera使其在废水处理系统中比其他细菌更具竞争力。