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Microwave-assisted aqueous two-phase system based extraction of phenolics from pulses: Antioxidant properties, characterization and encapsulation
Industrial Crops and Products ( IF 5.6 ) Pub Date : 2021-10-08 , DOI: 10.1016/j.indcrop.2021.114144
Elif Meltem İşçimen 1 , Mehmet Hayta 1
Affiliation  

In the current study, a microwave-assisted aqueous two-phase system (MA-ATPS) was examined for the extraction of phenolic compounds from green lentils, chickpeas, and dry beans. The recovery of phenolic compounds by MA-ATPS was optimized by response surface methodology (RSM). A comparative study was performed by conventional ATPS (C-ATPS) and classical mono-phase extraction (C). The DPPH scavenging activity, ABTS antioxidant activity, and metal chelating activity of the extracts were determined. The highest extraction yield (6.68, 2.10, and 1.69 mg GAE/ g sample for green lentil, chickpea and dry bean, respectively) and antioxidant properties (70.98, 7.60, and 9.68 μmol TE/g for DPPH scavenging activity for green lentil, chickpea, and dry bean respectively) were observed from the extracts obtained by MA-ATPS. Furthermore, the phenolic extracts were encapsulated by freeze-drying and the structural characterization of microcapsules has been performed by Fourier Transform Infrared (FT-IR) spectrometry and Scanning Electron Microscopy (SEM). The releasing profiles of the encapsulated phenolic compounds were investigated. Additionally, the effects of extracts on oxidative stability on sunflower oil under accelerated storage conditions were assessed. The results of the present study proved that the MA-ATPS might be considered as an efficient environment-friendly method for extraction phenolic compounds from pulses as evidenced by the antioxidant activity of extracts. Moreover, the encapsulated phenolic extracts may serve as an alternative ingredient for the food industry to be used in various functional formulations potentially leading to an increase in the value of green lentil, chickpea, and dry bean and possibly their by-products.



中文翻译:

基于微波辅助水性两相系统从脉冲中提取酚类物质:抗氧化特性、表征和封装

在当前的研究中,研究了微波辅助水性两相系统 (MA-ATPS) 从绿扁豆、鹰嘴豆和干豆中提取酚类化合物。MA-ATPS 对酚类化合物的回收率通过响应面法 (RSM) 进行了优化。通过传统的 ATPS (C-ATPS) 和经典的单相萃取 (C) 进行了比较研究。测定了提取物的DPPH清除活性、ABTS抗氧化活性和金属螯合活性。最高提取率(绿扁豆、鹰嘴豆和干豆分别为 6.68、2.10 和 1.69 mg GAE/g 样品)和抗氧化性能(绿扁豆、鹰嘴豆的 DPPH 清除活性为 70.98、7.60 和 9.68 μmol TE/g)和干豆)分别从 MA-ATPS 获得的提取物中观察到。此外,酚类提取物通过冷冻干燥封装,微胶囊的结构表征已通过傅里叶变换红外 (FT-IR) 光谱法和扫描电子显微镜 (SEM) 进行。研究了封装的酚类化合物的释放曲线。此外,还评估了提取物在加速储存条件下对葵花籽油氧化稳定性的影响。本研究的结果证明,MA-ATPS 可能被认为是一种从豆类中提取酚类化合物的有效的环保方法,提取物的抗氧化活性证明了这一点。而且,

更新日期:2021-10-08
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