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Improvement and validation of a computational model of flow in the swirling well cell culture model
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2021-10-06 , DOI: 10.1002/bit.27951 Mehwish Arshad 1, 2 , Ethan M Rowland 1 , Kai Riemer 1 , Spencer J Sherwin 2 , Peter D Weinberg 1
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2021-10-06 , DOI: 10.1002/bit.27951 Mehwish Arshad 1, 2 , Ethan M Rowland 1 , Kai Riemer 1 , Spencer J Sherwin 2 , Peter D Weinberg 1
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Effects of fluid dynamics on cells are often studied by growing the cells on the base of cylindrical wells or dishes that are swirled on the horizontal platform of an orbital shaker. The swirling culture medium applies a shear stress to the cells that varies in magnitude and directionality from the center to the edge of the vessel. Computational fluid dynamics methods are used to simulate the flow and hence calculate shear stresses at the base of the well. The shear characteristics at each radial location are then compared with cell behavior at the same position. Previous simulations have generally ignored effects of surface tension and wetting, and results have only occasionally been experimentally validated. We investigated whether such idealized simulations are sufficiently accurate, examining a commonly-used swirling well configuration. The breaking wave predicted by earlier simulations was not seen, and the edge-to-center difference in shear magnitude (but not directionality) almost disappeared, when surface tension and wetting were included. Optical measurements of fluid height and velocity agreed well only with the computational model that incorporated surface tension and wetting. These results demonstrate the importance of including accurate fluid properties in computational models of the swirling well method.
中文翻译：
旋流井细胞培养模型中流动计算模型的改进和验证
流体动力学对细胞的影响通常通过在圆柱形孔或盘子的底部培养细胞来研究，这些孔或盘子在轨道振荡器的水平平台上旋转。旋转培养基对细胞施加剪切应力，其大小和方向从容器中心到边缘变化。计算流体动力学方法用于模拟流动，从而计算井底的剪切应力。然后将每个径向位置的剪切特性与同一位置的细胞行为进行比较。以前的模拟通常忽略了表面张力和润湿的影响，结果只是偶尔经过实验验证。我们研究了这种理想化的模拟是否足够准确，检查了常用的旋流井配置。当包括表面张力和润湿时，没有看到早期模拟预测的破碎波，并且剪切幅度（但不是方向性）的边缘到中心差异几乎消失了。流体高度和速度的光学测量仅与结合了表面张力和润湿的计算模型一致。这些结果证明了在旋流井法的计算模型中包括准确的流体特性的重要性。
更新日期：2021-12-04
中文翻译：
旋流井细胞培养模型中流动计算模型的改进和验证
流体动力学对细胞的影响通常通过在圆柱形孔或盘子的底部培养细胞来研究，这些孔或盘子在轨道振荡器的水平平台上旋转。旋转培养基对细胞施加剪切应力，其大小和方向从容器中心到边缘变化。计算流体动力学方法用于模拟流动，从而计算井底的剪切应力。然后将每个径向位置的剪切特性与同一位置的细胞行为进行比较。以前的模拟通常忽略了表面张力和润湿的影响，结果只是偶尔经过实验验证。我们研究了这种理想化的模拟是否足够准确，检查了常用的旋流井配置。当包括表面张力和润湿时，没有看到早期模拟预测的破碎波，并且剪切幅度（但不是方向性）的边缘到中心差异几乎消失了。流体高度和速度的光学测量仅与结合了表面张力和润湿的计算模型一致。这些结果证明了在旋流井法的计算模型中包括准确的流体特性的重要性。
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