Chaotrope-Controlled Fabrication of Ferritin–Salvianolic Acid B- Epigallocatechin Gallate Three-Layer Nanoparticle by the Flexibility of Ferritin Channels,Journal of Agricultural and Food Chemistry

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Chaotrope-Controlled Fabrication of Ferritin–Salvianolic Acid B- Epigallocatechin Gallate Three-Layer Nanoparticle by the Flexibility of Ferritin Channels
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-10-06 , DOI: 10.1021/acs.jafc.1c01997
Shengnan Chen _{1,

2} , Yuqian Liu ₂ , Lei Zhu ₂ , Demei Meng _{1,

2} , Liqun Zhang ₂ , Qiaoe Wang ₃ , Jiangnan Hu ₂ , Desheng Wang ₄ , Zhiwei Wang ₂ , Zhongkai Zhou ₂ , Huanlu Song ₁ , Rui Yang _{1,

2}

Affiliation

Phytoferritin has a natural cagelike architecture for carrying bioactive molecules, and it is uniquely suited to function as a carrier due to its multiple interfaces and channels. In this study, a novel approach was proposed to prepare ferritin–salvianolic acid B-epigallocatechin gallate (EGCG) three-layer nanoparticles (FSE) through the steric hindrance of ferritin channels. Urea (30 mM) could expand the ferritin channel size evidenced by the improved iron release rate v_o and promote the EGCG penetration into the ferritin cavity without disassembly of the ferritin cage. The encapsulation ratio of EGCG was 16.0 ± 0.14% (w/w). Salvianolic acid B attached to the outer interface of ferritin through weak bonds with a binding constant of (2.91 ± 0.04) × 10⁵ M^–1. The FSE maintained a spherical structure with a diameter of 12 nm. Moreover, when subjected to heat (40–70 °C) there was a significant increase in the stability of EGCG in the FSE due to the binding of salvianolic acid B. Through this interesting approach, two molecules are simultaneously attached and encapsulated in ferritin in a multilayer form under moderate conditions, which is conducive to the protection of unstable molecules for potential encapsulation and delivery utilization.

中文翻译：

利用铁蛋白通道的灵活性离液控制制备铁蛋白-丹酚酸 B-表没食子儿茶素没食子酸酯三层纳米颗粒

植物铁蛋白具有用于携带生物活性分子的天然笼状结构，并且由于其多个界面和通道，它特别适合用作载体。在这项研究中，提出了一种通过铁蛋白通道的位阻制备铁蛋白-丹酚酸 B-表没食子儿茶素没食子酸酯 (EGCG) 三层纳米粒子 (FSE) 的新方法。尿素 (30 mM) 可以扩大铁蛋白通道尺寸，这由提高的铁释放速率v _o证明，并促进 EGCG 渗透到铁蛋白腔中，而无需拆卸铁蛋白笼。EGCG 的包封率为 16.0 ± 0.14% (w/w)。丹酚酸 B 通过弱键连接到铁蛋白的外界面，结合常数为 (2.91 ± 0.04) × 10 ⁵ M ^–1. FSE 保持了直径为 12 nm 的球形结构。此外，当受热（40-70°C）时，由于丹酚酸 B 的结合，FSE 中 EGCG 的稳定性显着增加。通过这种有趣的方法，两个分子同时附着并封装在铁蛋白中中等条件下的多层形式，有利于保护不稳定分子，用于潜在的封装和递送利用。

更新日期：2021-10-20

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