The pH parameter of soil plays a key role for plant nutrition as it is affecting the availability of minerals and consequently determines plant growth. Although the mechanisms by which root perceive the external pH is still unknown, the impact of external pH on tissue growth has been widely studied especially in hypocotyl and root. Thanks to technological development of cell imaging and fluorescent sensors, we can now monitor pH in real time with at subcellular definition. In this focus, fluorescent dye-based, as well as genetically-encoded pH indicators are discussed especially with respect to their ability to monitor acidic pH in the context of primary root. The notion of apoplastic subdomains is discussed and suggestions are made to develop fluorescent indicators for pH values below 5.0.

中文翻译：

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植物质外体中的 pH 生物传感——关注根细胞伸长

土壤的 pH 参数对植物营养起着关键作用，因为它会影响矿物质的可用性，从而决定植物的生长。尽管根部感知外部 pH 值的机制仍然未知，但外部 pH 值对组织生长的影响已被广泛研究，尤其是在下胚轴和根部。由于细胞成像和荧光传感器的技术发展，我们现在可以在亚细胞定义下实时监测 pH 值。在这个重点中，讨论了基于荧光染料以及基因编码的 pH 指示剂，特别是关于它们在初级根环境中监测酸性 pH 值的能力。讨论了质外体子域的概念，并提出了开发用于低于 5.0 的 pH 值的荧光指示剂的建议。