当前位置: X-MOL 学术Soil Biol. Biochem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Continuous in-situ measurement of free extracellular enzyme activity as direct indicator for soil biological activity
Soil Biology and Biochemistry ( IF 9.8 ) Pub Date : 2021-10-06 , DOI: 10.1016/j.soilbio.2021.108448
Ilil Levakov 1 , Zeev Ronen 1 , Hagar Siebner 1 , Ofer Dahan 1
Affiliation  

Understanding the nature and extent of biological activity in soils is critical for characterizing transformation processes, such as those involved in nutrient cycles and pollutant biodegradation. Unlike monitoring chemical and hydrological parameters, the current methods for analyzing microbial processes typically rely on destructive sampling, thus limiting our understanding of biogeochemical dynamics under rapidly changing environmental parameters. Extracellular enzymes are excellent representatives of the soil micro-fauna's response to changing conditions. We hypothesized that it is possible to analyze the activity of free enzymes passing with the soil pore-water through a suction cup porous interface. Thus, specific enzymes activity in the soil pore-water can provide information on the biological activity in the soil and unsaturated zone in a simple, non-destructive, continuous manner. The current study examined free enzyme activity in pore-water samples, as obtained from a porous interface during soil column experiments, as compared to values measured directly from the soil. The results demonstrated a high correlation between the measured enzymatic activities of phosphatase, β-glucosidase, and fluorescein diacetate (FDA) in the pore-water and the soil samples. Additionally, a preliminary implementation of the method was tested in ongoing remediation in a contaminated site. The results showed a strong correlation between the in-situ measurement of enzyme activity and the pollutant's biodegradation rate in the soil.



中文翻译:

连续原位测量游离细胞外酶活性作为土壤生物活性的直接指标

了解土壤中生物活动的性质和程度对于表征转化过程至关重要,例如那些涉及养分循环和污染物生物降解的过程。与监测化学和水文参数不同,目前分析微生物过程的方法通常依赖于破坏性采样,从而限制了我们对快速变化的环境参数下的生物地球化学动力学的理解。细胞外酶是土壤微型动物对变化条件的反应的极好代表。我们假设可以分析随土壤孔隙水通过吸盘多孔界面的游离酶的活性。因此,土壤孔隙水中的特定酶活性可以以简单、非破坏性、连续的方式提供有关土壤和非饱和带中生物活性的信息。目前的研究检查了孔隙水样品中的游离酶活性,这些样品是在土柱实验期间从多孔界面获得的,与直接从土壤中测量的值进行比较。结果表明,在孔隙水和土壤样品中测得的磷酸酶、β-葡萄糖苷酶和荧光素二乙酸酯 (FDA) 的酶活性之间存在高度相关性。此外,该方法的初步实施在受污染场地的持续修复中进行了测试。结果表明,酶活性的原位测量与土壤中污染物的生物降解率之间存在很强的相关性。连续方式。目前的研究检查了孔隙水样品中的游离酶活性,这些样品是在土柱实验期间从多孔界面获得的,与直接从土壤中测量的值进行比较。结果表明,在孔隙水和土壤样品中测得的磷酸酶、β-葡萄糖苷酶和荧光素二乙酸酯 (FDA) 的酶活性之间存在高度相关性。此外,该方法的初步实施在受污染场地的持续修复中进行了测试。结果表明,酶活性的原位测量与土壤中污染物的生物降解率之间存在很强的相关性。连续方式。目前的研究检查了孔隙水样品中的游离酶活性,这些样品是在土柱实验期间从多孔界面获得的,与直接从土壤中测量的值进行比较。结果表明,在孔隙水和土壤样品中测得的磷酸酶、β-葡萄糖苷酶和荧光素二乙酸酯 (FDA) 的酶活性之间存在高度相关性。此外,该方法的初步实施在受污染场地的持续修复中进行了测试。结果表明,酶活性的原位测量与土壤中污染物的生物降解率之间存在很强的相关性。与直接从土壤测量的值相比。结果表明,在孔隙水和土壤样品中测得的磷酸酶、β-葡萄糖苷酶和荧光素二乙酸酯 (FDA) 的酶活性之间存在高度相关性。此外,该方法的初步实施在受污染场地的持续修复中进行了测试。结果表明,酶活性的原位测量与土壤中污染物的生物降解率之间存在很强的相关性。与直接从土壤测量的值相比。结果表明,在孔隙水和土壤样品中测得的磷酸酶、β-葡萄糖苷酶和荧光素二乙酸酯 (FDA) 的酶活性之间存在高度相关性。此外,该方法的初步实施在受污染场地的持续修复中进行了测试。结果表明,酶活性的原位测量与土壤中污染物的生物降解率之间存在很强的相关性。

更新日期:2021-10-08
down
wechat
bug