A multistep approach was undertaken for biobutanol production targeting valorization of agricultural waste. Optimum production of lignocellulolytic enzymes [CMCase (3822.93U/mg), FPase (3640.93U/mg), β-glucosidase (3873.92U/mg), xylanase (3460.24U/mg), pectinase (3359.57U/mg), α-amylase (4136.54U/mg), and laccase (3863.16U/mg)] was accomplished through solid-substrate fermentation of pretreated mixed substrates (wheat bran, sugarcane bagasse and orange peel) by Aspergillus niger SKN1 and Trametes hirsuta SKH1. Partially purified enzyme cocktail was employed for saccharification of the said substrate mixture into fermentable sugar (69.23 g/L, product yield of 24% w/w). The recovered sugar with vegetable extract supplements was found as robust fermentable medium that supported 16.51 g/L biobutanol production by Clostridium acetobutylicum ATCC824. The sequential bioprocessing of low-priced substrates and exploitation of vegetable extract as growth factor for microbial butanol production will open a new vista in biofuel research.

为生物丁醇生产采取了多步骤方法，目标是农业废物的增值。木质纤维素分解酶的最佳产量 [CMCase (3822.93U/mg)、FPase (3640.93U/mg)、β-葡萄糖苷酶 (3873.92U/mg)、木聚糖酶 (3460.24U/mg)、果胶酶 (3359.57U)淀粉酶 (4136.54U/mg) 和漆酶 (3863.16U/mg)] 是通过黑曲霉SKN1 和毛栓菌对预处理的混合底物（麦麸、甘蔗渣和橙皮）进行固体底物发酵完成的SKH1。部分纯化的酶混合物用于将所述底物混合物糖化为可发酵糖（69.23 g/L，产物产率为24% w/w）。发现添加植物提取物的回收糖是稳定的可发酵培养基，支持丙酮丁醇梭菌ATCC824生产 16.51 g/L 的生物丁醇。低价底物的连续生物加工和植物提取物作为微生物丁醇生产的生长因子的开发将为生物燃料研究开辟新的前景。